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Fastkd3 (BC042506) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208992
Fastkd3 (tGFP-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $1,096.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fastkd3
Synonyms 2310010B21Rik; AW048587
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208992 representing BC042506
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTCATCACCCTGCGGAGGGCCTTTTGTCATAAGTCCATTCTTTGGATACCTGGAGCTGTGGTTG
CTCTGAAAATTCACCCTGCCAGTCATGCCCAAAAGGCGGTCACAGATCGCCTGAGTGTGTGCTTCTGCTC
CCTACAACCTGAGCTGTTCAGGGTCAGATTCCATCACGCATACTGTAAAAACTTCCACTCAGAAAAGGGA
AATGACTTTCATCCCGTGGGCGAGCCATGGTCCTCCCAGGCGCAGGAATGGAACCAGCCAGGACAGAGTC
TTCAAAACGAGGATGAAGAGATGCTTTTCAGGAGACTAAGCGTCTTCACTTCCTTTGAGGAAGTGCTGAG
TTTCATAAGTGCCCTGGACACCCTGCCTGTCCCTCTGGCAATGGCAGCTCTACTCAGGATCTGTGAGATA
GGGAGGAGAGATGGTGAGCAAAGGCTGCCAGAAGGAGTGCTGGAGAACCGTGCCTTCCAGGCTCTTTGCC
TCCGGTGTGAGCGAGACCCCTCACACCTGACCAGTGCTGGTTTGGTGACAGCTTTGCAATCTCTGCTTAC
GTTGTTGCCTGCAGATCCTCAAAGTAGCCTGATGCTGAGCCTGGTGGCAGAATGCCAACGTCGTCTCCAA
AGAGGCAACCTCGAAGTCCACCATCTTTGTGTCCTTGGGGAAAGTCTGGCCATGCTTCAAGGTGCGAGTT
GTGAGACCCTGAAGCTGGTTGTCCGTCAACTTCAAAGTAAAAGTGTGGAAACATTTGCTCCAGAGGAGAT
CGCGTCCGTTTATAGGATTCTGCAGGCGTGTCCTGAAGAAGTAGACAAACACCAGACGTTCTTAAACACA
TTAAACAACTTCTCCATTTCAGTAGTTCCCTACCTGAGCCCTAAATCCATTAGCCATGTCCTCACCGCCT
TAGTGGCCCTGGACCAGACTCACGCACTCCCACTGCTTATAAAGCTGGGCAAGTACGTTGTGAGATACAT
CCCCCGTTTTACCAACGAAGAGCTCAGGAAGGTCTTGGAGGCGTTTGTCTATTTCGGACACAGTGACAGG
TTCTTCACAGAGGCCTTAGAGCAACATGTGTCCGCCCTGTGCTTCTCCCTGGACCCCGCTGTGGCCAGCT
CCGTGATGGGGTACTGCAGCAGGAAACGGATTCTCTCCAAGCCCATCTTCGACGTAGTCTCAGAGATCGT
TGTTTGCCAGTGGGACAGACTTTCACCTAGTCAGATTGCCGAGCTAATTGAGCCATTTGGAAAGCTCAAT
TACGTGCCACCAAACGCCCCTGCCCTGTTTAGGAAGGTAGAAAATGTGCTCTGTGCCCGTTTACATCACT
TCCCACCAAAGATGCTACTGAGACTCCTTCATTCATGTGCGCTGATTGAGCGACATCCCGTCAACTTCAT
GTCAAAACTATTCAGCCCCTTTTTCCTTCAGCGGCTGCAAGGTAAAGAGTCTTATTTGGACAGATTGAGC
CTGGCACAGCTGACCCAACTTTTCCTAACCTCCGTCCTAGAGTGTCCGTTCTATAAGGGCCCCAAACTTC
TTCCTAAATATCAAGTGAAGTCATTTCTCACTCCATGCTGCTCCTTAGAGACGCCCATGGATCTCCATCT
GTATAAGTCTGTGGTGATTGGACTGATTGATCTTTTAGGATCAAGACTGTATTTTGCTTCAAAGGTGTTG
ACGCCCTATTACTACACTATAGCCTCGGCCCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208992 representing BC042506
Red=Cloning site Green=Tags(s)

MAFITLRRAFCHKSILWIPGAVVALKIHPASHAQKAVTDRLSVCFCSLQPELFRVRFHHAYCKNFHSEKG
NDFHPVGEPWSSQAQEWNQPGQSLQNEDEEMLFRRLSVFTSFEEVLSFISALDTLPVPLAMAALLRICEI
GRRDGEQRLPEGVLENRAFQALCLRCERDPSHLTSAGLVTALQSLLTLLPADPQSSLMLSLVAECQRRLQ
RGNLEVHHLCVLGESLAMLQGASCETLKLVVRQLQSKSVETFAPEEIASVYRILQACPEEVDKHQTFLNT
LNNFSISVVPYLSPKSISHVLTALVALDQTHALPLLIKLGKYVVRYIPRFTNEELRKVLEAFVYFGHSDR
FFTEALEQHVSALCFSLDPAVASSVMGYCSRKRILSKPIFDVVSEIVVCQWDRLSPSQIAELIEPFGKLN
YVPPNAPALFRKVENVLCARLHHFPPKMLLRLLHSCALIERHPVNFMSKLFSPFFLQRLQGKESYLDRLS
LAQLTQLFLTSVLECPFYKGPKLLPKYQVKSFLTPCCSLETPMDLHLYKSVVIGLIDLLGSRLYFASKVL
TPYYYTIASAL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC042506
ORF Size 1713 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC042506, AAH42506
RefSeq Size 2522 bp
RefSeq ORF 1715 bp
Locus ID 69577
Cytogenetics 13 B3
Summary Required for normal mitochondrial respiration. Increases steady-state levels and half-lives of a subset of mature mitochondrial mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1 mRNA translation and increases mitochondrial complex IV assembly and activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC042506" in other vectors (4)
SKU Description Size Price
MC217862 Fastkd3 (untagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412), (10ug) 10 ug
$586.00
MR208992 Fastkd3 (Myc-DDK-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412) 10 ug
$589.00 $896.00
MR208992L3 Lenti ORF clone of Fastkd3 (Myc-DDK-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412) 10 ug
$1,196.00
MR208992L4 Lenti ORF clone of Fastkd3 (mGFP-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412) 10 ug
$1,196.00

Plasmid Map for MG208992

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208992
Circular map for MG208992

file-alt Citations

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