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Pak3 (NM_008778) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208677
Pak3 (tGFP-tagged) - Mouse p21 (CDKN1A)-activated kinase 3 (Pak3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $907.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pak3
Synonyms PAK-3; Pak65alpha; Pak65beta; Stk4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008778
ORF Size 8222 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008778.3, NP_032804.2
RefSeq Size 2172 bp
RefSeq ORF 1635 bp
Locus ID 18481
UniProt ID Q61036
Cytogenetics X F2
Summary Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity (PubMed:25851601). Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.UniProtKB/Swiss-Prot Function
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"NM_008778" in other vectors (5)
SKU Description Size Price
MC204741 Pak3 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 4, (10ug) 10 ug
$507.00
MG225428 Pak3 (tGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3) transcript variant 4, (10ug) 10 ug
$707.00
MR225428 Pak3 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 4 10 ug
$289.00 $507.00
MR225428L3 Lenti ORF clone of Pak3 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 4 10 ug
$807.00
MR225428L4 Lenti ORF clone of Pak3 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 4 10 ug
$807.00

Plasmid Map for MG208677

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Cloning scheme for MG208677
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