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Zyx (BC054775) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208545
Zyx (tGFP-tagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $696.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zyx
Synonyms 9530098H06Rik; R75157
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208545 representing BC054775
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCCCCCGCCCGCCTCCCGCGATCTCCGTCTCCGTCTCGGCCCCCGCGTTTTACGCCCCGCAGA
AGAAGTTCGCCCCGGTTGTGGCCCCAAAGCCCAAAGTGAATCCTTTCCGGCCTGGGGACAGCGAGCCTCC
TGTAGCAGCCGGGGCCCAAAGAGCGCAGATGGGTCGGGTGGGCGAGATCCCACCACCACCCCCGGAAGAC
TTTCCTTTGCCCCCTCCTCCCCTTATTGGGGAGGGCGACGACTCAGAGGGTGCCCTGGGAGGTGCCTTCC
CACCTCCACCTCCCCCGATGATCGAGGAACCATTCCCCCCTGCTCCTCTGGAGGAGGACATCTTCCCCTC
CCCTCCACCTCCACTGGAGGAGGAGGGAGGGCCTGAGGCCCCTACCCAGCTCCCACCGCAGGTATCATCC
GGATATGTACCCCCACCAGTTGCCACTCCATTTGTTCCCAAGCCTAGTACCAAACCTGCCCCTGGGGGCA
CAGCACCCTTGCCTCCTTGGAAGACCCCTTCTAGCTCCCAGCCACCACCTCAGCCGCAGGCCAAGCCTCA
GGTCCAGCTCCATGTCCAGCCTCAGGCCAAGCCCCATGTCCAACCCCAGCCTGTGTCTTCTGCTAATACA
CAGCCCCGGGGTCCCCTTTCTCAGGCACCAACTCCAGCACCTAAGTTTGCTCCAGTGGCTCCTAAATTTA
CTCCCGTGGTTTCCAAGTTCAGCCCTGGTGCTCCAAGTGGACCTGGGCCACAGCCCAATCAAAAAATGGT
GCCTCCGGATGCTCCTTCTTCTGTGAGCACAGGCTCCCCTCAGCCCCCTAGCTTCACCTATGCTCAGCAG
AAGGAGAAGCCCCTAGTTCAAGAGAAGCAGCACCCACAGCCTCCACCAGCTCAAAACCAAAACCAGGTAC
GCTCTCCTGGAGGCCCAGGCCCCTTGACCCTGAAGGAGGTAGAGGAGTTGGAGCAGCTGACCCAGCAGCT
GATGCAGGACATGGAACACCCTCAGAGGCAGAGCGTGGCAGTGAATGAGTCCTGTGGCAAATGCAATCAG
CCACTGGCCCGTGCACAGCCTGCGGTTCGTGCACTGGGACAACTGTTCCACATCACCTGCTTCACTTGCC
ATCAGTGTCAGCAGCAGCTGCAGGGACAGCAGTTCTATAGCCTGGAGGGAGCACCATATTGTGAGGGCTG
CTACACCGACACTTTGGAGAAGTGCAACACCTGTGGGCAGCCCATCACTGACCGCATGCTGAGGGCCACT
GGCAAAGCCTACCACCCACAGTGCTTCACCTGTGTGGTCTGCGCCTGTCCCCTGGAGGGCACCTCCTTCA
TTGTGGACCAGGCCAATCAGCCCCACTGTGTCCCTGACTATCACAAGCAATACGCTCCAAGGTGCTCCGT
CTGCTCGGAGCCAATCATGCCTGAGCCTGGCCGAGACGAGACTGTGCGAGTAGTGGCGCTGGATAAGAAC
TTTCATATGAAGTGTTACAAGTGTGAGGACTGTGGGAAACCTCTGTCCATTGAGGCAGATGACAACGGCT
GTTTCCCTCTGGATGGCCACGTCCTTTGTCGGAAGTGCCACTCCGCTAGAGCCCAGACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208545 representing BC054775
Red=Cloning site Green=Tags(s)

MAAPRPPPAISVSVSAPAFYAPQKKFAPVVAPKPKVNPFRPGDSEPPVAAGAQRAQMGRVGEIPPPPPED
FPLPPPPLIGEGDDSEGALGGAFPPPPPPMIEEPFPPAPLEEDIFPSPPPPLEEEGGPEAPTQLPPQVSS
GYVPPPVATPFVPKPSTKPAPGGTAPLPPWKTPSSSQPPPQPQAKPQVQLHVQPQAKPHVQPQPVSSANT
QPRGPLSQAPTPAPKFAPVAPKFTPVVSKFSPGAPSGPGPQPNQKMVPPDAPSSVSTGSPQPPSFTYAQQ
KEKPLVQEKQHPQPPPAQNQNQVRSPGGPGPLTLKEVEELEQLTQQLMQDMEHPQRQSVAVNESCGKCNQ
PLARAQPAVRALGQLFHITCFTCHQCQQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGQPITDRMLRAT
GKAYHPQCFTCVVCACPLEGTSFIVDQANQPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKN
FHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHSARAQT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC054775
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC054775, AAH54775
RefSeq Size 2323 bp
RefSeq ORF 1601 bp
Locus ID 22793
Cytogenetics 6 B2.1
Summary Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"BC054775" in other vectors (4)
SKU Description Size Price
MC217768 Zyx (untagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149), (10ug) 10 ug
$547.00
MR208545 Zyx (Myc-DDK-tagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149) 10 ug
$289.00 $496.00
MR208545L3 Lenti ORF clone of Zyx (Myc-DDK-tagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149) 10 ug
$796.00
MR208545L4 Lenti ORF clone of Zyx (mGFP-tagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149) 10 ug
$796.00

Plasmid Map for MG208545

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208545
Circular map for MG208545

file-alt Citations

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