Ankrd13d (NM_026720) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG208296
Ankrd13d (tGFP-tagged) - Mouse ankyrin repeat domain 13 family, member D (Ankrd13d)
  $683.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd13d
Synonyms 0710001P18Rik; AI430801
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208296 representing NM_026720
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTACAGTTGGTACTCCAGTATCGGGACTTCCAGAGGGCCACACAGAGGTTGGCTGGCATTCCAGAAC
TGCTCAACAAACTCCGTCAGGCCCCTGATTTCTACGTGGAAATGAAATGGGAGTTCACTAGCTGGGTACC
TCTTGTGTCCAAGATGTGCCCGAGCGATGTGTACCGTGTGTGGAAGCGGGGTGAGAGTCTGCGGGTGGAC
ACTAGTCTCTTGGGCTTTGAGCACATGACCTGGCAGCGTGGCCGAAGGAGCTTCATCTTCAGGGGTCAGG
AGGCGGGAGCCTTGGTGATGGAAGTGGATCATGACCGGCAGGTGGTGCACACAGAGACCCTAGCTCCAGC
TCTGCATGAACCAGAAGCGCTGCTGGCTGCCATGCGGCCTAGTGAGGAGCACGTGGCCAGTCGGCTCACC
TCTCCTATTGTCTCCACCCACCTGGACACTCGCAATGTAGCCTTTGAGAGGAACAAATGTGGTATCTGGG
GATGGCGGTCTGAGAAGATGGAGTCTGTTAGTGGCTACGAGGCAAAGGTGTACAGTGCCACCAATGTGGA
GCTGGTGACACGCACAAGAACAGAACACCTCTCTGACCAGGACAAGTTAAGGAACAAAGGGGGGAAGACT
CCATTCCAGTCCTTCCTGGGCATGGCCCAGCAGCACTCCTCCCACACTTTGGCTCCCGTGCAGCAGGCAG
CCAGCCCCACCAACCCTACAGCTATCTCTGCTGAGGAATACTTTGACCCCAGCTTCAGCCTGGAATCCAG
AAACATTGGCCGCCCCATTGAGATGTCTAGCAAAGTGCAGAGGTTCAAGGCAACACTGTGGCTAAGTGAG
GAGCACCCACTGTCCTTGGGTGACCAGGTGACACCCATCATTGACCTGATGGCCATCAGCAACGCTCATT
TTGCCAAGCTACGGGATTTCATCACTCTGCGTCTCCCACCAGGCTTCCCTGTCAAGATTGAGATTCCCCT
CTTCCATGTGCTCAATGCCCGCATCACCTTCAGTAACCTTTGCGGCTGTGATGAGCCTGTGAGCTCGGTG
TGCGTCCCCAACTCCAGTTCTGCCATTTCTGCTTCAGGAAGCCCTTTTCCATGTGAGGTGGACCCCACTG
TATTCGAGGTGCCCGAGGGATACAGTGTGCTGGGTGCTGAGCGCAGCGAGCCCCTTCGGGATGAGGATGA
CGACCTGCTGCAGTTTGCCATCCAGCAGAGCCTGCTTGAGGCAGGCACAGAGGCAGAGCAGGTGACGGTG
TGGGAAGCCCTGACCAACACACGGCCTGGCATTCACCCTCCTCCCCGAGTCACAGTGTTTGAAGAGCAGC
TTCAGTTGGAGCAGGCCCTGCAGGAAAGCCTACAGCTGTCCACAGAGTCCAGGGGTCCAGAATCTCCTCA
GAAGACACCTCCATCCCCTGCACCCCCAAGCTTTGAGGAGCAGCTTCGCCTGGCCCTGGAGTTGTCTTCA
AGGGAACAGGAGGAGTTGGAGCGACGGGGTCAGCAGGAAGAAGACGACTTACAGCGGATCCTGCAGCTGT
CACTCACAGAGCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208296 representing NM_026720
Red=Cloning site Green=Tags(s)

MVQLVLQYRDFQRATQRLAGIPELLNKLRQAPDFYVEMKWEFTSWVPLVSKMCPSDVYRVWKRGESLRVD
TSLLGFEHMTWQRGRRSFIFRGQEAGALVMEVDHDRQVVHTETLAPALHEPEALLAAMRPSEEHVASRLT
SPIVSTHLDTRNVAFERNKCGIWGWRSEKMESVSGYEAKVYSATNVELVTRTRTEHLSDQDKLRNKGGKT
PFQSFLGMAQQHSSHTLAPVQQAASPTNPTAISAEEYFDPSFSLESRNIGRPIEMSSKVQRFKATLWLSE
EHPLSLGDQVTPIIDLMAISNAHFAKLRDFITLRLPPGFPVKIEIPLFHVLNARITFSNLCGCDEPVSSV
CVPNSSSAISASGSPFPCEVDPTVFEVPEGYSVLGAERSEPLRDEDDDLLQFAIQQSLLEAGTEAEQVTV
WEALTNTRPGIHPPPRVTVFEEQLQLEQALQESLQLSTESRGPESPQKTPPSPAPPSFEEQLRLALELSS
REQEELERRGQQEEDDLQRILQLSLTEH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026720
ORF Size 1554 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026720.1, NP_080996.1
RefSeq Size 2113 bp
RefSeq ORF 1818 bp
Locus ID 68423
UniProt ID Q6PD24
Cytogenetics 19 A
Summary Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026720" in other vectors (4)
SKU Description Size Price
MC203411 Ankrd13d (untagged) - Mouse ankyrin repeat domain 13 family, member D (Ankrd13d), (10ug) 10 ug
$483.00
MR208296 Ankrd13d (Myc-DDK-tagged) - Mouse ankyrin repeat domain 13 family, member D (Ankrd13d) 10 ug
$289.00 $483.00
MR208296L3 Lenti ORF clone of Ankrd13d (Myc-DDK-tagged) - Mouse ankyrin repeat domain 13 family, member D (Ankrd13d) 10 ug
$783.00
MR208296L4 Lenti ORF clone of Ankrd13d (mGFP-tagged) - Mouse ankyrin repeat domain 13 family, member D (Ankrd13d) 10 ug
$783.00

Plasmid Map for MG208296

Download sequence for

Cloning scheme for MG208296

Circular map for MG208296

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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