Mycn (NM_008709) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG207382
Mycn (tGFP-tagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn)
$489.00 $886.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mycn
Synonyms bHLHe37; c-nmyc; N-myc; Nmyc; Nmyc-1; Nmyc1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207382 representing NM_008709
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAGCTGCACCGCGTCCACCATGCCGGGGATGATCTGCAAGAACCCAGACCTCGAGTTTGACTCAC
TGCAGCCCTGCTTCTACCCGGACGAAGATGACTTCTACTTCGGCGGTCCCGACTCGACCCCACCGGGGGA
GGACATCTGGAAGAAGTTTGAGCTGCTGCCCACGCCCCCGTTGTCGCCCAGCCGCGCCTTCCCAGAGCAC
AGCCCGGAGCCTTCGAATTGGGCTACGGAGATGCTGCTGCCGGAGGCCGACCTGTGGGGCAACCCGGCCG
AGGAGGATGCGTTCGGTCTCGGGGGCCTGGGTGGCCTCACTCCTAATCCGGTCATCCTTCAGGACTGCAT
GTGGAGCGGCTTCTCTGCCCGCGAGAAGCTAGAGCGCGCAGTGAACGAAAAACTACAGCACGGCCACGGG
CCCCCGGGCGTCAGCTCAGCCTGCTCGGCTCCCGGAGTGGGTGCCAGCAGCCCCGGGGGCCGTGCCCTTG
GTGGGTCGTCGAGTGCTAGCCACACCGGGGCCACCCTGCCTACCGACCTCTCCCACCCGGCTGCCGAATG
TGTGGACCCCGCCGTGGTCTTCCCCTTCCCGGTGAACAAGCGAGAGTCGGCGTCGGTGCCCGCTGCCCCC
ACTAGCGCCCCGGCGACCAGCGCTGCGGTCACTAGTGTGTCTGTTCCAGCTACTGCCCCGGTGGCTGCTC
CTGCTCGTGCAGGCGGCCGTCCTGCCAGCAGTGGGGAGGCCAAGGCCCTCAGCACCTCCGGAGAGGATAC
CTTGAGCGACTCAGATGATGAGGATGACGAGGAGGAAGATGAAGAGGAGGAAATCGATGTGGTCACCGTA
GAGAAGAGACGTTCCTCCTCTAACAACAAGGCGGTAACCACTTTCACGATCACTGTGCGTCCCAAGACCT
CCGCTCTGGGCCTGGGGCGAGCACAGCCTGGCGAGCTGATCCTCAAGCGCTGTGTTCCCATCCATCAGCA
GCACAACTATGCTGCACCCTCACCCTACGTGGAGAGCGAGGACGCGCCCCCGCAGAAAAAGATCAAGAGC
GAGGCTTCTCCACGCCCCCTCAAAAGTGTTGTTCCAGCAAAAGCGAAGAGCCTGAGCCCCCGAAACTCAG
ACTCGGAGGACAGCGAGCGCCGCCGCAACCACAACATCCTGGAGCGTCAACGCCGGAACGACCTGCGCTC
CAGCTTCCTGACGCTCAGGGACCATGTGCCTGAGCTGGTGAAGAACGAGAAGGCCGCCAAGGTGGTCATC
TTGAAAAAGGCCACCGAGTACGTGCACGCCCTACAGGCCAACGAGCACCAGCTCCTGCTGGAAAAGGAGA
AACTGCAGGCGAGGCAGCAGCAGTTGCTAAAGAAGATCGAACACGCTCGGACTTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207382 representing NM_008709
Red=Cloning site Green=Tags(s)

MPSCTASTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRAFPEH
SPEPSNWATEMLLPEADLWGNPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVNEKLQHGHG
PPGVSSACSAPGVGASSPGGRALGGSSSASHTGATLPTDLSHPAAECVDPAVVFPFPVNKRESASVPAAP
TSAPATSAAVTSVSVPATAPVAAPARAGGRPASSGEAKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTV
EKRRSSSNNKAVTTFTITVRPKTSALGLGRAQPGELILKRCVPIHQQHNYAAPSPYVESEDAPPQKKIKS
EASPRPLKSVVPAKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVI
LKKATEYVHALQANEHQLLLEKEKLQARQQQLLKKIEHARTC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008709
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008709.3, NP_032735.3
RefSeq Size 2596 bp
RefSeq ORF 1389 bp
Locus ID 18109
UniProt ID P03966
Cytogenetics 12 6.14 cM
Summary Positively regulates the transcription of MYCNOS in neuroblastoma cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008709" in other vectors (6)
SKU Description Size Price
MC205645 Mycn (untagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn), (10ug) 10 ug
$686.00
MR207382 Mycn (Myc-DDK-tagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn) 10 ug
$289.00 $686.00
MR207382L1 Lenti ORF clone of Mycn (Myc-DDK-tagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn) 10 ug
$986.00
MR207382L2 Lenti ORF clone of Mycn (mGFP-tagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn) 10 ug
$986.00
MR207382L3 Lenti ORF clone of Mycn (Myc-DDK-tagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn) 10 ug
$986.00
MR207382L4 Lenti ORF clone of Mycn (mGFP-tagged) - Mouse v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn) 10 ug
$986.00

Plasmid Map for MG207382

Download sequence for

Cloning scheme for MG207382

Circular map for MG207382

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