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BC024139 (BC063071) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206228
BC024139 (tGFP-tagged) - Mouse cDNA sequence BC024139 (cDNA clone MGC:67439 IMAGE:5694464)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol BC024139
Synonyms 6230424I18, MGC37978
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206228 representing BC063071
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGGCTTGGTGTGGGAGTCTGTGGGGTGTCACAGGCTCAGTGCCTTGTGTTTGTTCACAGGAGG
TGTGTGAGGGGGTTGTGGCAAGCACCAGGGACCTGGAAAGGGTTTTGCAGACCGGCCAGAGGCTGGCAGA
GCTTCTCAGTAGTGATCAAGCTCGGCTTGTACGACAGCAGCTGAATCAGTTCCAGGAGCGTGTCAAGCTG
ACTGAATCACAAGCCACCTGTGCCCGGCAGAAGCTACTATTTGCTCAAAGGACAGAGTCCTCCAAGCCCT
TACCACCGGCAGGCCTGGAGGCCCAGCTTGAACTGGAAGGCTCTGCCTCAACACCTCCACAGCTCAAGGA
TCTGAAGCAGCTGAGTGTCCAGCTCGTCCAGTTAGCAGAACGACTTGAGCAGCTGGCTCAGTGGGCAGAG
ACTTCTAGTCAGGTAGCAGCACCCGCAGTGACCATCTGGGAACAGAGTCTCTCATCAGCCATGCTTCAGG
CCGATACGAAGCCCCTGGAAGGGCACTGGTTAGAGACTCGAGAACAAGACTTTGGGCCGAAGGCCATATG
GGAGCCAGCTGTGCAGATCTCATGTGGCCTGGCAGATCAGGCAGCCATTGAGAAGCTAAAGCCTGAGAGG
GACTGGAACCCAAGTTTACAGGAGTTCCTGTGGGAACAGCAAACCCTGGCAGTGTCATGGCTAGGAAGGA
GAGGAGCTGCTGACTATGGGAGCACCTGCCACTTGGGCCTGCTGATGGACCTGCAGGGCTGCCGGGCTGC
CATGTCTAGCCCCTCCTGCGAGAGGATACACACCCTGGAAGGGGTGCTGCTGCTTGTGGGCCACCTTGTA
GAGAAGTTGGTCTTGTGCCCCCATAGACTGAGCCAAGCGGAACCCTTGGGGATCAAGGTGGTACAAGCCC
ACAGCTTGTTAGAGGAGATGCCTCCGGCCCTCACCCCAAAGTACTGGACTCGAGCCCCAAGGGAGATACT
GGCTTTGATGAGTCTTGACCAGGAGCCGCCTGGGGATCTGGCGAGTAGAGCTAAGGCTTGCCTCCTGGGG
AAGATTCCAAAAGAACTGAGCTGGAGGCCATGGTGGTCAGAGGTCATGGAGAACTGGAGTCTTAGGAAGC
CAGAGCATAGCAAGAGGAGCGAGGACAAAAGGGACCAGTGGGAACGGCTGGAACAGGGCCACCAGGTACA
G

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206228 representing BC063071
Red=Cloning site Green=Tags(s)

MAQAWCGSLWGVTGSVPCVCSQEVCEGVVASTRDLERVLQTGQRLAELLSSDQARLVRQQLNQFQERVKL
TESQATCARQKLLFAQRTESSKPLPPAGLEAQLELEGSASTPPQLKDLKQLSVQLVQLAERLEQLAQWAE
TSSQVAAPAVTIWEQSLSSAMLQADTKPLEGHWLETREQDFGPKAIWEPAVQISCGLADQAAIEKLKPER
DWNPSLQEFLWEQQTLAVSWLGRRGAADYGSTCHLGLLMDLQGCRAAMSSPSCERIHTLEGVLLLVGHLV
EKLVLCPHRLSQAEPLGIKVVQAHSLLEEMPPALTPKYWTRAPREILALMSLDQEPPGDLASRAKACLLG
KIPKELSWRPWWSEVMENWSLRKPEHSKRSEDKRDQWERLEQGHQVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC063071
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC063071, AAH63071
RefSeq Size 2407 bp
RefSeq ORF 1193 bp
Locus ID 271278
Cytogenetics 15 D3
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"BC063071" in other vectors (4)
SKU Description Size Price
MC218046 BC024139 (untagged) - Mouse cDNA sequence BC024139 (cDNA clone MGC:67439 IMAGE:5694464), (10ug) 10 ug
$503.00
MR206228 BC024139 (Myc-DDK-tagged) - Mouse cDNA sequence BC024139 (cDNA clone MGC:67439 IMAGE:5694464) 10 ug
$289.00 $457.00
MR206228L3 Lenti ORF clone of BC024139 (Myc-DDK-tagged) - Mouse cDNA sequence BC024139 (cDNA clone MGC:67439 IMAGE:5694464) 10 ug
$757.00
MR206228L4 Lenti ORF clone of BC024139 (mGFP-tagged) - Mouse cDNA sequence BC024139 (cDNA clone MGC:67439 IMAGE:5694464) 10 ug
$757.00

Plasmid Map for MG206228

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG206228
Circular map for MG206228

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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