Rad9a (NM_011237) Mouse Tagged ORF Clone
$657.00
3 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Rad9a |
| Synonyms | Rad9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011237 |
| ORF Size | 1167 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011237.2, NP_035367.1 |
| RefSeq Size | 2046 bp |
| RefSeq ORF | 1170 bp |
| Locus ID | 19367 |
| UniProt ID | Q9Z0F6 |
| Cytogenetics | 19 A |
| Summary | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011237" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR206078 | Rad9a (Myc-DDK-tagged) - Mouse RAD9 homolog (S. pombe) (Rad9) | 10 ug |
$289.00
$457.00
|
|
| MR206078L3 | Lenti ORF clone of Rad9a (Myc-DDK-tagged) - Mouse RAD9 homolog (S. pombe) (Rad9) | 10 ug |
$757.00
|
|
| MR206078L4 | Lenti ORF clone of Rad9a (mGFP-tagged) - Mouse RAD9 homolog (S. pombe) (Rad9) | 10 ug |
$757.00
|
|
Citations
The CDK-PLK1 axis targets the DNA damage checkpoint sensor protein RAD9 to promote cell proliferation and tolerance to genotoxic stress
Wakida, Ikura, Kuriya et al
Elife (2017) 6 DOI: 10.7554/eLife.29953
Wakida, Ikura, Kuriya et al
Elife (2017) 6 DOI: 10.7554/eLife.29953
AI Summary: OriGene contributed to this study by providing the RAD9A cDNA, which was used to construct tagged RAD9 expression plasmids for investigating the regulation of the DNA damage checkpoint.
Snippet: The RAD9A cDNA was purchased from OriGene. The gene encoding C-terminally myc-His6 (mH)-tagged RAD9 was constructed in pCDNA3.1 or pCDNA5/FRT
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
