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Gm13152 (BC066875) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206012
(tGFP-tagged) - Mouse cDNA clone MGC:76795 IMAGE:30111586
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm13152
Synonyms OTTMUSG00000010671
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206012 representing BC066875
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGTGTCTCTGCTAAACATGCCACAGGGTTTGTTATCCTTCAAGGACGTGGCCTTGGACTTCTCAC
TGGAGGAATGGGAATGTCTCAGTTTTGCTCAGAGGTCATTGTACATGGATGTGATGTTGGAGGATTACAA
CAATCTGTTGTTTGTGGAAAATCATTGCATACATGGAAAGTATGAGAAGGTCATGGATCAAGACAGCCAG
TACATTGTTCTTGAACACATGAATATTCAAGAGAAGTCTTCTAAATGGAACAAGCTTAGCAATGTGATTC
TAGAATCCTCCCATTATACACCTCACAAATGTACTGAATGGGACAAATGCTTTTCCCAAAAATCCCATCT
TAATTTTCATCAGAAAATTCATGCAGGAGAGAAATCTTACAAATGCAGTGAATGCGACAAATGCTTTACC
GAAAAAGGCAGTCTCAGAATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAAATGTAGCGAATGTG
ACAAATGCTTTACTGGAAAAGGCAATCTGAGAATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAA
ATGCAGTGAATGTGACAAATGCTTTACCAAACAATCCAATCTTAGTATTCATCAGAGAATTCATACAGGA
GAGAAACCTTACAAATGCAGTGAATGTGACAAATGCTTTACCCAAGAATCCTATCTTAGTATTCATCAGA
GAATTCATACAGGAGAGAAACCTTACAAATGCAGTGAATGTGGCAAATGCTTTACTGAAAAAAGCAGTCT
GAGAATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAAATGCAGTGAATGTGACAAATGCTTTACC
CAACAATCCCATCTTAGTATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAAATGCAGTGAATGTG
ACAAATGCTTTACCCAACAATCCAATCTTAGTATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAA
ATGCACTGAATGTGGCAAATGCTTTACTGAAAAAAGCAGTCTGAGAATTCATCAGAGAATTCATACAGGA
GAGAAACCTTACAAATGCAGTGAATGTGGCAAATGCTTTACTGAAAAAAAGCAGTCTGAGAATTCATCAG
AGAATTCATACAGGAGAGAAACCTTACAAATGCAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206012 representing BC066875
Red=Cloning site Green=Tags(s)

MSVSLLNMPQGLLSFKDVALDFSLEEWECLSFAQRSLYMDVMLEDYNNLLFVENHCIHGKYEKVMDQDSQ
YIVLEHMNIQEKSSKWNKLSNVILESSHYTPHKCTEWDKCFSQKSHLNFHQKIHAGEKSYKCSECDKCFT
EKGSLRIHQRIHTGEKPYKCSECDKCFTGKGNLRIHQRIHTGEKPYKCSECDKCFTKQSNLSIHQRIHTG
EKPYKCSECDKCFTQESYLSIHQRIHTGEKPYKCSECGKCFTEKSSLRIHQRIHTGEKPYKCSECDKCFT
QQSHLSIHQRIHTGEKPYKCSECDKCFTQQSNLSIHQRIHTGEKPYKCTECGKCFTEKSSLRIHQRIHTG
EKPYKCSECGKCFTEKKQSENSSENSYRRETLQMQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC066875
ORF Size 1155 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC066875, AAH66875
RefSeq Size 2891 bp
RefSeq ORF 1157 bp
Locus ID 195531
Cytogenetics 4
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"BC066875" in other vectors (3)
SKU Description Size Price
MR206012 (Myc-DDK-tagged) - Mouse cDNA clone MGC:76795 IMAGE:30111586 10 ug
$289.00 $457.00
MR206012L3 Lenti ORF clone of MGC:76795 (Myc-DDK-tagged) - Mouse cDNA clone MGC:76795 IMAGE:30111586 10 ug
$757.00
MR206012L4 Lenti ORF clone of MGC:76795 (mGFP-tagged) - Mouse cDNA clone MGC:76795 IMAGE:30111586 10 ug
$757.00

Plasmid Map for MG206012

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MG206012
Circular map for MG206012

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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