B3gntl1 (BC088993) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG205305
B3gntl1 (tGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 (cDNA clone
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol B3gntl1
Synonyms 6030413G23Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205305 representing BC088993
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTCTCCGAGAACGCCACAACGCGCGGCTCGCTGGCCCTAGTGTCTATCATCCTCCCCGTGCACA
ACGCTGAGCAGTGGTTGGATGAGTGCTTGATGTCTGTTTTGCAGCAAGACTTTGAAGGTGCCATGGAGCT
CTCTGTTTTTAACGATGCCAGCAAGGACAAGTCTAGGGCCATCATTGAAAAATGGAAGGTGAAACTGGAA
GATTCTGGAATCTCTGTAGTCATCGGAGGCCATGACTCTCCTTCTCCCAGAGGAGTTGGATATTCTAAAA
ACCAAGCCGTCGCACAGAGCACAGGGTCTTACCTTTGCTTTTTGGATTCAAGAGTGAGGATGCAGTACGA
AGCTGCCGGTCAGCACCCGACGAGTATCATAGGATGCCAAGTGAGAAGAGATCCCCCGGACTCTACAGAG
CGATATACACGTTGGATCAACCATTTGACATCTGATCAGCTGCTGACACAGGTGTTCACCTCCCACGGCC
CCACGGTGATCATGCCTACCTGGTTCTGCTCTCGGGCCTGGTTCTCCCACGTGGGCCCATTTGATGAAGG
AGGCCAGGGTGTTCCAGAGGATCTGCTGTTCTTCTATGAGCACCTCAGGAAGGGCGGTGGCGTCTTCCGG
GTGGACCACAGCCTGCTACTCTACCGATACCACCTGTATGCAGCCACACATTCGGTCCTTGAGATGACCA
TCTGGACGCACCGTGTCCACTTCCTAGAGGAACAAGTCCTACCTCACTGGAAGTCCTTCACCATCTGGAA
TGCAGGCAAGCAGGGGCGTAAGTTATACCGCAGCCTGACTGCAGCCAGCCGGCACAAGGTGGTGGCCTTC
TGTGACGTTGATGAGAACAAGATCAGGAAGGGCTTCTACTGCTACGAAGACTCACAGGAAAGACCCAAGC
CAAAGGTCCCCATCCTGCATTTCCAAGCTGCCCAGTCTCCCTTTGTCATCTGTGTGAAGCTGGACCTCAC
AGGAGGTGAGTTTGAAGACAACTTGAAGTCGCTGGACTTGCAGGAAGGCCGGGACTTCGTCCATTTCAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205305 representing BC088993
Red=Cloning site Green=Tags(s)

MEVSENATTRGSLALVSIILPVHNAEQWLDECLMSVLQQDFEGAMELSVFNDASKDKSRAIIEKWKVKLE
DSGISVVIGGHDSPSPRGVGYSKNQAVAQSTGSYLCFLDSRVRMQYEAAGQHPTSIIGCQVRRDPPDSTE
RYTRWINHLTSDQLLTQVFTSHGPTVIMPTWFCSRAWFSHVGPFDEGGQGVPEDLLFFYEHLRKGGGVFR
VDHSLLLYRYHLYAATHSVLEMTIWTHRVHFLEEQVLPHWKSFTIWNAGKQGRKLYRSLTAASRHKVVAF
CDVDENKIRKGFYCYEDSQERPKPKVPILHFQAAQSPFVICVKLDLTGGEFEDNLKSLDLQEGRDFVHFS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC088993
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC088993, AAH88993
RefSeq Size 2734 bp
RefSeq ORF 1052 bp
Locus ID 210004
Cytogenetics 11 E2
Summary Putative glycosyltransferase.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC088993" in other vectors (4)
SKU Description Size Price
MC217970 B3gntl1 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 (cDNA clone, (10ug) 10 ug
$503.00
MR205305 B3gntl1 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 (cDNA clone 10 ug
$289.00 $457.00
MR205305L3 Lenti ORF clone of B3gntl1 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 (cDNA clone 10 ug
$757.00
MR205305L4 Lenti ORF clone of B3gntl1 (mGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 (cDNA clone 10 ug
$757.00

Plasmid Map for MG205305

Download sequence for

Cloning scheme for MG205305

Circular map for MG205305

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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