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H2 (BC080812) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205115
H2 (tGFP-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol H2
Synonyms Q1b, Q1d, Q1k
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205115 representing BC080812
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACTGGGAAGGCTGCTCCTGCTGCTGGCAGCCGCCCTGACCCTGACCAAAACCGGAGCGGGCTCAC
ACTCGCTGCGGTATTTCGAGACCTCGGTGTCCCAGCCCGGCTTCGGGAAGCCCCGGTTCATCTCTGTCGG
CTACGTGGACGACACGCAGTTTGTGCGCTTCGACAGCGACGCGAAGAATCCGAGATATGAGCTGCGGGCG
CCGTGGATGGAGCAGGAGGGGCCGGAGTATTGGGAGCGGAACACACGGAGAGTCAAGGGCAGTGAGAAGA
GATTCCAAGAGAGCCTGAGCACCCTGCTCAGCTACTACAACCAGAGCAAGGGCGGCATTCACACCTTCCA
GAAGTTGTCTGGCTGTGATCTGGGGTCAGATGGGCGCCTTCAAAGCGGGTACCTGCAGTTCGCCTATGAT
GGCCTTGATTACATCGCCCTGAATGAAGACCTGGAAACCTGGACAGCAGCAGATGTGGCAGCTCAGGAAA
CCCGACACAAGTGGGAGCAGGCTGGTGCTGCTGAGAAACACAGGACCTACCTGGAGGGCAAGTGCCTGAT
GTGGCTCCACAGATACCTGGAGCTCAGGAAGGAGATGCTGCTGCGCACAGATCCCCCAAAGGCACATGTG
ACTCATCACCCCAGATCTCAAGGTGATGTCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCTGACA
TCACCCTGACCTGGCAGTTGAATGGGGAGGAGCTGACCCAGCACATGGAGCTTGTGGAGACCAGGCCTGC
AGGGGATGGAACCTTCCAGAAGTGGGCATCTGTGGTGGTGCCTCTTGGGAAGGAGCAGAATTACACATGC
CATGTGTACCATGAGGGGCTGCCTGAGCCCCTCACCCTGAGATGGGAGCCTCCTCCATACACTGTTTCCA
ACATGGTAATCATAGCTGTTCTGGTTGTCCTTGGAGCTGTGATAGTCATTGGAGCTGTGGTCATCATTGG
AGTTATGGTGTCTTTTGTGATGAAGAGGAGGAGAAACAAAGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205115 representing BC080812
Red=Cloning site Green=Tags(s)

MALGRLLLLLAAALTLTKTGAGSHSLRYFETSVSQPGFGKPRFISVGYVDDTQFVRFDSDAKNPRYELRA
PWMEQEGPEYWERNTRRVKGSEKRFQESLSTLLSYYNQSKGGIHTFQKLSGCDLGSDGRLQSGYLQFAYD
GLDYIALNEDLETWTAADVAAQETRHKWEQAGAAEKHRTYLEGKCLMWLHRYLELRKEMLLRTDPPKAHV
THHPRSQGDVTLRCWALGFYPADITLTWQLNGEELTQHMELVETRPAGDGTFQKWASVVVPLGKEQNYTC
HVYHEGLPEPLTLRWEPPPYTVSNMVIIAVLVVLGAVIVIGAVVIIGVMVSFVMKRRRNKA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC080812
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC080812, AAH80812
RefSeq Size 2001 bp
RefSeq ORF 1025 bp
Locus ID 15006
Cytogenetics 17 18.63 cM
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"BC080812" in other vectors (4)
SKU Description Size Price
MC218176 H2 (untagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887), (10ug) 10 ug
$503.00
MR205115 H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887) 10 ug
$289.00 $457.00
MR205115L3 Lenti ORF clone of H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887) 10 ug
$757.00
MR205115L4 Lenti ORF clone of H2 (mGFP-tagged) - Mouse histocompatibility 2, Q region locus 1 (cDNA clone MGC:91064 IMAGE:6398887) 10 ug
$757.00

Plasmid Map for MG205115

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205115
Circular map for MG205115

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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