Ybx1 (BC031472) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG204659
Ybx1 (tGFP-tagged) - Mouse Y box protein 1 (cDNA clone MGC:25376 IMAGE:4167717)
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ybx1 |
| Synonyms | 1700102N10Rik, MSY1, mYB-1a, EF1A, YB-1, dbpB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC031472 |
| ORF Size | 966 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC031472, AAH31472 |
| RefSeq Size | 1562 bp |
| RefSeq ORF | 968 bp |
| Locus ID | 22608 |
| Cytogenetics | 4 D2.1 |
| Summary | Mediates pre-mRNA alternative splicing regulation. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds preferentially to 5'-CUCUGCG-3' motif in vitro (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1
Kishikawa, Otsuka, Yoshikawa et al
Nat Commun (2016) 7, 13006 DOI: 10.1038/ncomms13006
Kishikawa, Otsuka, Yoshikawa et al
Nat Commun (2016) 7, 13006 DOI: 10.1038/ncomms13006
Cited by 64
AI Summary: OriGene products pCMV6-mYBX1-cDNA-GFP (MG204659) and pCMV6kan-mYBX1-cDNA (MC204526) were used to overexpress YBX1 protein and generate YBX1-GFP fusion constructs, enabling the study of YBX1’s role in pancreatic oncogenic processes driven by satellite RNAs.
Snippet: Using this plasmid as a template, PCR-amplified HA-tagged YBX1 ORF was subcloned into the pCDH-CMV-MCS-EF1-Neo vector (pCDH-HA-YBX1). To express YBX1 protein conjugated with GFP protein (pLVSIN-YBX1-GFP), the pCMV6-mYBX1-cDNA-GFP vector (MG204659, OriGene) was purchased and subcloned into the pLVSIN-EF1a neo vector (TaKaRa) at the BamHI site using the InFusion HD cloning kit. For the overexpression of YBX1 protein, the pCMV6kan-mYBX1-cDNA vector (MC204526) was purchased from OriGene Technologies (Rockville, MD, USA)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
