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Pycr2 (NM_133705) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204629
Pycr2 (tGFP-tagged) - Mouse pyrroline-5-carboxylate reductase family, member 2 (Pycr2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pycr2
Synonyms 1810018M05Rik; P5cr2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204629 representing NM_133705
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGTGGGTTTCATCGGCGCAGGCCAGCTGGCCTGTGCTCTGGCGCGGGGCTTCACGGCCGCGGGCG
TCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCGGATATGGACCTGCCCACGGTGTCTGCGCTCAGGAG
GATGGGTGTGAATCTGACCCGAAGCAATAAGGACACTGTGCGGCACAGCGACGTCCTGTTCCTGGCTGTG
AAGCCCCACATCATCCCCTTCATCCTGGATGAGATTGGAGCCGACGTGCAGGAGAGGCACATCGTGGTCT
CCTGTGCAGCCGGGGTCACCATCAGTTCTGTGGAGAAGAAGCTGATGGCTTTCCAGCCGGCCCCCAAAGT
GATCCGCTGCATGACCAACACGCCCGTGGTGGTCCGAGAGGGGGCCACCGTGTATGCCACGGGCACCCAT
GCTCTGGTGGAAGACGGCAAGCTTTTGGAGCAGCTGATGAGCAGTGTGGGCTTCTGCACAGAGGTGGAGG
AAGACCTCATTGATGCTATCACCGGGCTCAGCGGCAGCGGGCCTGCCTATGCATTCATGGCCCTGGACGC
CCTGGCAGATGGTGGGGTGAAGATGGGCGTGCCGCGGCGCCTGGCGGTCAGACTGGGAGCTCAGGCGTTG
CTGGGAGCAGCTAAGATGCTGCTGGACTCAGAGGACCATCCAGGCCAGCTCAAGGACAACGTCTGCTCCC
CTGGGGGGGCCACCATCCATGCCCTGCACTTCCTGGAGAGTGGGGGCTTCCGCTCTCTGCTCATCAATGC
GGTTGAGGCCTCCTGTATCCGAACCAGAGAACTGCAGTCCATGGCTGACCAAGAAAAGGTGTCTCCTGCT
GCCCTTAAGAAGACCCTTCTGGACAGAGTGAAGCTGGAGTCACCCACGGTGTCCACGCTGGCCCCACCCA
GCTCAGGGAAACTCCTTACAAGAAACCCCGCCCAGGGAAGCAAGAAGGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204629 representing NM_133705
Red=Cloning site Green=Tags(s)

MSVGFIGAGQLACALARGFTAAGVLSAHKIIASSPDMDLPTVSALRRMGVNLTRSNKDTVRHSDVLFLAV
KPHIIPFILDEIGADVQERHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTH
ALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGLSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQAL
LGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKVSPA
ALKKTLLDRVKLESPTVSTLAPPSSGKLLTRNPAQGSKKE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133705
ORF Size 960 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133705.2
RefSeq Size 1590 bp
RefSeq ORF 963 bp
Locus ID 69051
UniProt ID Q922Q4
Cytogenetics 1 H4
Summary Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (By similarity). Involved in cellular response to oxidative stress (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_133705" in other vectors (3)
SKU Description Size Price
MR204629 Pycr2 (Myc-DDK-tagged) - Mouse pyrroline-5-carboxylate reductase family, member 2 (Pycr2) 10 ug
$289.00 $300.00
MR204629L3 Lenti ORF clone of Pycr2 (Myc-DDK-tagged) - Mouse pyrroline-5-carboxylate reductase family, member 2 (Pycr2) 10 ug
$600.00
MR204629L4 Lenti ORF clone of Pycr2 (mGFP-tagged) - Mouse pyrroline-5-carboxylate reductase family, member 2 (Pycr2) 10 ug
$600.00

Plasmid Map for MG204629

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG204629
Circular map for MG204629

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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