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Dedd2 (NM_207677) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204459
Dedd2 (tGFP-tagged) - Mouse death effector domain-containing DNA binding protein 2 (Dedd2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dedd2
Synonyms 2410050E11Rik; FLAME-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204459 representing NM_207677
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTGTCCGGGTCGACCCCGGCCCCGAGCTGGGAGGAGGATGAGTGTTTGGATTACTACGGCATGC
TGTCGCTTCACCGTATGTTCGAGGTAGTGGGCGGGCAGCTGACCGAGTGCGAACTGGAGCTCCTGGCCTT
CCTGCTGGATGAGGCCCCCGGCGCCCCCGGCGGCCTGGCCCGCGCCCGCAGCGGCCTGGAGCTGCTTCTG
GAGCTGGAACGCCGCGGGCAGTGCGACGAGAGCAACCTGCGGCTGCTGAGCCAACTCCTGCGCGTGCTAG
CCCGTCACGACCTCCTGCCACATCTGGCGCGCAAGCGGCGCCGGCCAGTGTCCCCTGAGCGATACAGCTA
TGGCAATCCCAGCTCTTCTTCCAAGAGGACAGAGGACAGTTGCCGGCGTCGGCGACAGGCGAGCAGTTCT
TCGGACTCTCCTCAGAGTCAGTGGGACACAGGCTCCCCACCAACCAAGCGGCAGCGGCGGAGTCGGGGCC
GCCCTAGCAGTGGTGCCCGGCAGCGGCGGAGAGCAGGCCTTGCAGCCTCGCAGCAGCACCAGCAGCACCA
GGAGTTGGGCAGGCCCTCCTCAGAAGGCAAGGTGACCTGTGACATCCGGCTCCGGGTTCGGGCAGAGTAC
TGCGAGCATGGGCCAGCCTTGGAGCAGGGTGTGGCGTCCCGGAGACCTCAGGCGTTGGCCCGGCAGCTGG
ATGTGTTTGGACAAGCCACTGCTGTGCTGCGGTCAAGGGACCTGGGCTCTGTGGTGTGTGACATCAAGTT
CTCAGAGCTGTCCTATCTGGACGCCTTCTGGGGCGACTACCTGAGTGGCGCCCTGCTGCAGGCCCTTCGG
GGCGTGTTCCTGACTGAGGCTTTACGGGAGGCCGTGGGCCGAGAGGCTGTCCGCTTGCTGGTCAGTGTGG
ACGAGGCTGACTATGAGGCTGGCCGGCGTCGCCTGTTGCTGATGGAGGAGGAAGGAGGGCGGCGTGGGAC
CGAGGCCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204459 representing NM_207677
Red=Cloning site Green=Tags(s)

MALSGSTPAPSWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAPGGLARARSGLELLL
ELERRGQCDESNLRLLSQLLRVLARHDLLPHLARKRRRPVSPERYSYGNPSSSSKRTEDSCRRRRQASSS
SDSPQSQWDTGSPPTKRQRRSRGRPSSGARQRRRAGLAASQQHQQHQELGRPSSEGKVTCDIRLRVRAEY
CEHGPALEQGVASRRPQALARQLDVFGQATAVLRSRDLGSVVCDIKFSELSYLDAFWGDYLSGALLQALR
GVFLTEALREAVGREAVRLLVSVDEADYEAGRRRLLLMEEEGGRRGTEAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207677
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207677.3, NP_997560.3
RefSeq Size 1828 bp
RefSeq ORF 993 bp
Locus ID 67379
UniProt ID Q8QZV0
Cytogenetics 7 A3
Summary May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.UniProtKB/Swiss-Prot Function
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"NM_207677" in other vectors (4)
SKU Description Size Price
MC210543 Dedd2 (untagged) - Mouse death effector domain-containing DNA binding protein 2 (Dedd2), (10ug) 10 ug
$330.00
MR204459 Dedd2 (Myc-DDK-tagged) - Mouse death effector domain-containing DNA binding protein 2 (Dedd2) 10 ug
$289.00 $300.00
MR204459L3 Lenti ORF clone of Dedd2 (Myc-DDK-tagged) - Mouse death effector domain-containing DNA binding protein 2 (Dedd2) 10 ug
$600.00
MR204459L4 Lenti ORF clone of Dedd2 (mGFP-tagged) - Mouse death effector domain-containing DNA binding protein 2 (Dedd2) 10 ug
$600.00

Plasmid Map for MG204459

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG204459
Circular map for MG204459

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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