Mtch2 (NM_019758) Mouse Tagged ORF Clone

SKU MG204242

Mtch2 (tGFP-tagged) - Mouse mitochondrial carrier homolog 2 (C. elegans) (Mtch2), nuclear gene encoding mitochondrial protein

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Mtch2
• Synonyms: 2310034D24Rik; 4930539J07Rik; HSPC0; HSPC032
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204242 representing NM_019758; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGACGCGGCCAGTCAGGTGCTCCTGGGCTCCGGTCTCACCATCCTGTCCCAGCCGCTCATGTACG; TGAAAGTGCTCATCCAGGTGGGATATGAGCCTCTTCCTCCAACAATAGGACGAAATATTTTTGGGCGACA; AGTATGTCAGCTTCCTGGCCTCTTTTGCTATGCTCAGCACATTGCAAGCATCGATGGGAGGCGTGGGTTG; TTCACAGGCTTGACTCCAAGACTGTGTTCAGGAGTCCTTGGAACTGTGGTCCATGGGAAAGTCTTACAGT; ATTACCAGGAGTCTGAGAAACCTGAGGAGTTAGGATCTGTAACTGTACAAAAAGAATATTCATCCTCCTT; TGACCGAGTTATCAAAGAGACAACTCGAGAGATGATTGCTCGTTCTGCTGCTACCCTCATTACACATCCC; TTCCACGTGATCACTCTGAGGTCCATGGTACAGTTTATTGGCAGAGAGTCTAAGTACTGTGGACTGTGTG; ACTCCATAGTAACCATCTACCGGGAAGAAGGCATCGTAGGATTTTTTGCGGGTCTCATTCCTCGCCTCCT; AGGTGACATCATTTCTTTGTGGCTGTGTAACTCACTGGCCTATCTCATCAATACCTATGCACTGGACAGT; GGGGTTTCTACCATGAATGAAATGAAAAGTTACTCCCAAGCTGTCACAGGATTCTTTGCCAGTATGTTGA; CATATCCCTTTGTGCTTGTATCTAATCTTATGGCCGTCAACAACTGTGGGCTTGCTGGTGGATCTCCTCC; TTATTCCCCAATATACACTTCTTGGATAGATTGCTGGTGCATGCTACAAAAAGCGGGAAATATGAGCCGA; GGAAACAGCTTGTTTTTCCGGAAGGTTCCTTGTGGGAAGACTTACTGTTATGACCTAAGAATGTTAATC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204242 representing NM_019758; Red=Cloning site Green=Tags(s); ; MADAASQVLLGSGLTILSQPLMYVKVLIQVGYEPLPPTIGRNIFGRQVCQLPGLFCYAQHIASIDGRRGL; FTGLTPRLCSGVLGTVVHGKVLQYYQESEKPEELGSVTVQKEYSSSFDRVIKETTREMIARSAATLITHP; FHVITLRSMVQFIGRESKYCGLCDSIVTIYREEGIVGFFAGLIPRLLGDIISLWLCNSLAYLINTYALDS; GVSTMNEMKSYSQAVTGFFASMLTYPFVLVSNLMAVNNCGLAGGSPPYSPIYTSWIDCWCMLQKAGNMSR; GNSLFFRKVPCGKTYCYDLRMLI; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019758
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019758.3
• RefSeq Size: 2420 bp
• RefSeq ORF: 912 bp
• Locus ID: 56428
• UniProt ID: Q791V5
• Cytogenetics: 2 E1
• Summary: This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target that can produce two isoforms from the same mRNA by use of alternative in-frame translation termination codons. [provided by RefSeq, Dec 2017]