Decr2 (NM_011933) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG204004
Decr2 (tGFP-tagged) - Mouse 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal (Decr2)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Decr2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204004 representing NM_011933
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGCCGCCGCCCGACGTTGAGGGGGATGACTGTCTTCCTGAATACCACCATCTCTTCTGCCCGG
ATCTTCTCCAGGACAAAGTGGCCTTTATCACAGGTGGTGGTTCTGGGATTGGCTTCCGGATCGCAGAGAT
TTTCATGAGGCATGGCTGCCACACTGTCATCGTCGGCAGGAGTCTGCAGAAAGTGACCACGGCTGCTAAG
AAGTTGGTTGCTGCCACCGGAAAGCGGTGCCTCCCTCTGTCTATGGATGTCCGAGTTCCCCCAGAGGTCA
TGACTGCTGTGGATCAAGCGCTGCAAGAATTTGGCAAAATCAACATCCTCATTAACTGTGCAGCTGGAAA
CTTCCTATGCCCTGCCAGTGCTTTGTCTTTCAATGCCTTTAAGACCGTGGTTGACATTGACACCATCGGT
ACCTTCAATGTGTCTAGTGTGCTTTACAAGAAGTTCTTCCGGGACCATGGAGGAGTGATTGTGAACATTA
CTGCCACCCTCAGTATGAGGGGGCAGGTGCTACAGCTACATGCAGGCGCTGCCAAAGCAGCTGTGGATGC
TATGACGCGACACTTGGCTGTGGAATGGGGTCCCCAGAATATCCGTGTCAACAGCCTGGCTCCTGGTGCC
ATCAGTGGCACTGAGGGTTTAAGGCGATTAAGAGGCTCCAATGCCAGTTCGAAATTAAAGCATTTTTCAA
ATCCTATTCCAAGACTTGGGACCAAGACAGAGATCGCTCACAGCGTGCTGTACCTGGCCAGCCCTCTGGC
TTCCTATGTCTCAGGTATCGTGCTGGTGGTTGATGGTGGAAGCTGGATGACATTCCCCAATGGCATCAAG
CAACTGCTAGAGTTTGAATCCTTCTCTGCTAAGCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204004 representing NM_011933
Red=Cloning site Green=Tags(s)

MAQPPPDVEGDDCLPEYHHLFCPDLLQDKVAFITGGGSGIGFRIAEIFMRHGCHTVIVGRSLQKVTTAAK
KLVAATGKRCLPLSMDVRVPPEVMTAVDQALQEFGKINILINCAAGNFLCPASALSFNAFKTVVDIDTIG
TFNVSSVLYKKFFRDHGGVIVNITATLSMRGQVLQLHAGAAKAAVDAMTRHLAVEWGPQNIRVNSLAPGA
ISGTEGLRRLRGSNASSKLKHFSNPIPRLGTKTEIAHSVLYLASPLASYVSGIVLVVDGGSWMTFPNGIK
QLLEFESFSAKL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011933
ORF Size 876 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011933.2, NP_036063.1
RefSeq Size 2051 bp
RefSeq ORF 879 bp
Locus ID 26378
UniProt ID Q9WV68
Cytogenetics 17 13.06 cM
Summary Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011933" in other vectors (4)
SKU Description Size Price
MC209714 Decr2 (untagged) - Mouse 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal (Decr2), (10ug) 10 ug
$330.00
MR204004 Decr2 (Myc-DDK-tagged) - Mouse 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal (Decr2) 10 ug
$289.00 $300.00
MR204004L3 Lenti ORF clone of Decr2 (Myc-DDK-tagged) - Mouse 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal (Decr2) 10 ug
$600.00
MR204004L4 Lenti ORF clone of Decr2 (mGFP-tagged) - Mouse 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal (Decr2) 10 ug
$600.00

Plasmid Map for MG204004

Download sequence for

Cloning scheme for MG204004

Circular map for MG204004

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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