Tra2b (NM_009186) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG203931
Tra2b (tGFP-tagged) - Mouse splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (Sfrs10)
  $530.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tra2b
Synonyms 5730405G21Rik; D16Ertd266e; Sfrs10; SIG-41; Silg41; TRA2beta
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203931 representing NM_009186
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGACAGCGGCGAGCAGAACTACGGCGAGCGGGAATCCCGTTCTGCTTCCCGAAGTGGAAGTGCTC
ACGGATCGGGGAAATCTGCACGGCATACACCTGCAAGGTCTCGCTCCAAGGAAGACTCAAGGCGTTCTAG
ATCAAAGTCCAGGTCCAGGTCTGAATCTAGGTCTAGATCCAGAAGAAGTTCTAGAAGGCATTATACAAGG
TCACGATCACGATCTCGCTCGCATAGACGATCCCGGAGCAGGTCTTACAGCCGAGATTATCGCAGGCGCC
ACAGCCACAGCCATTCTCCCATGTCTACTCGAAGGCGTCATGTTGGGAACCGGGCAAATCCTGACCCCAA
CTGTTGTCTTGGCGTGTTTGGGTTGAGCTTATACACCACAGAAAGAGACCTAAGAGAAGTGTTCTCTAAA
TATGGCCCCATTGCTGATGTGTCTATTGTATATGACCAGCAATCTAGACGTTCAAGAGGATTTGCCTTTG
TATATTTTGAAAATGTAGACGATGCCAAGGAAGCTAAAGAACGTGCCAATGGAATGGAGCTTGATGGGCG
TCGAATTAGAGTCGATTTCTCTATAACAAAAAGGCCCCATACCCCAACACCAGGAATTTATATGGGGAGA
CCCACTTATGGCAGTTCTCGCCGCCGAGACTATTATGACAGAGGGTACGATCGGGGTTATGATGACCGGG
ACTATTACAGCAGATCATACAGAGGAGGAGGTGGTGGAGGAGGTGGATGGAGAGCAGCTCAAGACAGGGA
TCAGATTTACAGAAGACGGTCACCTTCTCCTTACTACAGTCGTGGAGGATACAGGTCACGTTCTCGATCA
CGATCCTACTCACCTCGTCGCTAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203931 representing NM_009186
Red=Cloning site Green=Tags(s)

MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTR
SRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSK
YGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGR
PTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRS
RSYSPRRY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009186
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009186.5
RefSeq Size 2135 bp
RefSeq ORF 867 bp
Locus ID 20462
UniProt ID P62996
Cytogenetics 16 13.18 cM
Summary Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA (By similarity).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009186" in other vectors (4)
SKU Description Size Price
MC204978 Tra2b (untagged) - Mouse transformer 2 beta homolog (Drosophila) (Tra2b), (10ug) 10 ug
$300.00
MR203931 Tra2b (Myc-DDK-tagged) - Mouse transformer 2 beta homolog (Drosophila) (Tra2b) 10 ug
$289.00 $330.00
MR203931L3 Lenti ORF clone of Tra2b (Myc-DDK-tagged) - Mouse transformer 2 beta homolog (Drosophila) (Tra2b) 10 ug
$630.00
MR203931L4 Lenti ORF clone of Tra2b (mGFP-tagged) - Mouse transformer 2 beta homolog (Drosophila) (Tra2b) 10 ug
$630.00

Plasmid Map for MG203931

Download sequence for

Cloning scheme for MG203931

Circular map for MG203931

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products