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Ube2q1 (BC082275) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG203239
Ube2q1 (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 1 (cDNA clone MGC:90946 IMAGE:6310111)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ube2q1
Synonyms NICE-5, PRO3094, Ube2q
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203239 representing BC082275
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGATCAGCCCTTGCCAGCAGAGCAGTGCACGCAGGAAGAAGTGTCTTCAGAAGATGAAGATGAAG
AGATGCCTGAGGATACAGAAGACCTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCCGAGGGCAAGAA
GTCTGAAGATGATGGCATCGGAAAAGAGAACCTGGCCATCCTAGAGAAAATTAAAAAGAACCAGAGGCAA
GATTACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATA
TATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTCTCTATGACTG
GAATGTCAAACTCCTCAAAGTTGACCAGGATAGCGCTTTGCACAATGATCTTCAGATCCTGAAGGAGAAG
GAAGGAGCAGACTTCATCCTACTTAACTTCTCCTTTAAAGATAACTTCCCCTTTGACCCACCGTTCGTCA
GGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGTGGAGGTGCCATCTGCATGGAACTTCTCAC
CAAGCAGGGCTGGAGCAGTGCCTACTCCATAGAGTCCGTGATCATGCAGATCAGCGCCACGCTGGTGAAG
GGGAAAGCACGGGTGCAGTTTGGAGCCAACAAATCTCAGTATAGCCTGACGAGAGCACAGCAGTCCTACA
AGTCCTTGGTGCAGATCCATGAAAAAAACGGCTGGTACACACCCCCAAAGGAAGATGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203239 representing BC082275
Red=Cloning site Green=Tags(s)

MLDQPLPAEQCTQEEVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKSEDDGIGKENLAILEKIKKNQRQ
DYLNGAVSGSVQATDRLMKELRDIYRSQSFKGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEK
EGADFILLNFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIESVIMQISATLVK
GKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPKEDG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC082275
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC082275, AAH82275
RefSeq Size 1686 bp
RefSeq ORF 761 bp
Locus ID 70093
Cytogenetics 3 F1
Summary Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Involved in female fertility and embryo implantation (PubMed:23108111). May be involved in hormonal homeostasis in females (PubMed:23108111). Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation (PubMed:18511602).UniProtKB/Swiss-Prot Function
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"BC082275" in other vectors (4)
SKU Description Size Price
MC217869 Ube2q1 (untagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 1 (cDNA clone MGC:90946 IMAGE:6310111), (10ug) 10 ug
$330.00
MR203239 Ube2q1 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 1 (cDNA clone MGC:90946 IMAGE:6310111) 10 ug
$289.00 $300.00
MR203239L3 Lenti ORF clone of Ube2q1 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 1 (cDNA clone MGC:90946 IMAGE:6310111) 10 ug
$600.00
MR203239L4 Lenti ORF clone of Ube2q1 (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 1 (cDNA clone MGC:90946 IMAGE:6310111) 10 ug
$600.00

Plasmid Map for MG203239

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MG203239
Circular map for MG203239

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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