Cldn2 (NM_016675) Mouse Tagged ORF Clone
$489.00
$500.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cldn2 |
| Synonyms | AL022813 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016675 |
| ORF Size | 690 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016675.4 |
| RefSeq Size | 3042 bp |
| RefSeq ORF | 693 bp |
| Locus ID | 12738 |
| UniProt ID | O88552 |
| Cytogenetics | X F1 |
| Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The knockout mice lacking this gene display normal appearance, activity, growth and behavior, but are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules. The proteins encoded by this gene and another family member Cldn12 are also critical for vitamin D-dependent Ca2+ absorption between enterocytes. provided by RefSeq, Aug 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016675" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR202768 | Cldn2 (Myc-DDK-tagged) - Mouse claudin 2 (Cldn2) | 10 ug |
$289.00
$300.00
|
|
| MR202768L3 | Lenti ORF clone of Cldn2 (Myc-DDK-tagged) - Mouse claudin 2 (Cldn2) | 10 ug |
$600.00
|
|
| MR202768L4 | Lenti ORF clone of Cldn2 (mGFP-tagged) - Mouse claudin 2 (Cldn2) | 10 ug |
$600.00
|
|
Plasmid Map for MG202768
Download sequence for
Cloning scheme for MG202768
Circular map for MG202768
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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