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Tbn (BC057895) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202540
Tbn (tGFP-tagged) - Mouse taube nuss (cDNA clone MGC:68125 IMAGE:5031867)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tbn
Synonyms AW260255; Tbn
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202540 representing BC057895
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACACGGCGGCCGGGCCTGGTGGCTCCGGAACGAGACCGGGAAGTAAGCAATCCACTAACCCTG
CTGATAACTACCATCTGGCCCGGAGGAGGACCCTGCAAGTGGTTGTGAGCTCCTTGCTGACAGAGGCGGG
ATTTGAGAGTGCCGAGAAAGCGTCTGTGGAGACACTGACAGAGATGCTGCAGAGCTACATCTCAGAAATC
GGGAGAAGTGCTAAGTCATACTGTGAGCACACAGCCAGGACCCAGCCCACACTGTCGGACATCGTGGTCA
CATTGGTTGAAATGGGCTTCAATGTTGACACTCTACCTGCCTATGCAAAACGGTCTCAGAGGATGGTCAT
CACTGCACCTCCAGTGACCAATCAGCCAGTGACCCCCAAGGCCCTCACTGCAGGGCAGAACCGGCCCCAC
CCGCCACACATCCCCAGCCATTTTCCTGAGTTTCCGGATCCCCACACCTACATCAAAACTCCGACATACC
GTGAGCCAGTGTCTGACTACCAGATCCTTCGGGAGAAGGCCGCATCTCAGAGACGAGACGTGGAGCGAGC
ACTCACCCGCTTCATGGCCAAGACAGGCGAGACCCAGAGTCTTTTCAAGGATGATGTCAGCACTTTTCCT
TTGATCTCTGACCAGCATTTGATTAAAGCACCG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202540 representing BC057895
Red=Cloning site Green=Tags(s)

MADTAAGPGGSGTRPGSKQSTNPADNYHLARRRTLQVVVSSLLTEAGFESAEKASVETLTEMLQSYISEI
GRSAKSYCEHTARTQPTLSDIVVTLVEMGFNVDTLPAYAKRSQRMVITAPPVTNQPVTPKALTAGQNRPH
PPHIPSHFPEFPDPHTYIKTPTYREPVSDYQILREKAASQRRDVERALTRFMAKTGETQSLFKDDVSTFP
LISDQHLIKAP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC057895
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC057895, AAH57895
RefSeq Size 1618 bp
RefSeq ORF 665 bp
Locus ID 63856
Cytogenetics 17 C
Summary Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex (By similarity). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC057895" in other vectors (4)
SKU Description Size Price
MC206887 Tbn (untagged) - Mouse taube nuss (cDNA clone MGC:68125 IMAGE:5031867), (10ug) 10 ug
$330.00
MR202540 Tbn (Myc-DDK-tagged) - Mouse taube nuss (cDNA clone MGC:68125 IMAGE:5031867) 10 ug
$289.00 $300.00
MR202540L3 Lenti ORF clone of Tbn (Myc-DDK-tagged) - Mouse taube nuss (cDNA clone MGC:68125 IMAGE:5031867) 10 ug
$600.00
MR202540L4 Lenti ORF clone of Tbn (mGFP-tagged) - Mouse taube nuss (cDNA clone MGC:68125 IMAGE:5031867) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.