Mob3b (NM_178061) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202388
Mob3b (tGFP-tagged) - Mouse MOB1, Mps One Binder kinase activator-like 2B (yeast) (Mobkl2b)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mob3b
Synonyms 8430436F23Rik; A430018A01Rik; Mobkl2b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202388 representing NM_178061
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATCGCCCTGAAGCAAGTGTTCAACAAGGACAAGACCTTCCGGCCCAAGAGGAAGTTTGAACCCG
GCACACAAAGATTTGAGCTGCACAAACGAGCTCAGGCGTCCCTCAACTCGGGTGTGGACCTGAGGGCAGC
CGTGCAGTTACCCAACGGCGAGGACCAGAACGACTGGGTGGCTGTGCACGTGGTGGACTTCTTCAATCGC
ATTAACCTCATCTACGGCACCATCTGTGAGTTCTGCACTGAGCGGACCTGCCCTGTGATGTCAGGGGGCC
CGAAATACGAGTACCGATGGCAGGATGACCTCAAGTATAAGAAACCAACTGCACTGCCCGCTCCCCAGTA
CATGAATCTGCTTATGGATTGGATTGAAGTCCAGATAAACAATGAGGACATATTTCCAACATGTGTTGGC
GTCCCCTTCCCAAAGAACTTCCTCCAGATCTGCAAGAAGATCCTATGCCGCCTCTTTCGGGTCTTCGTCC
ATGTCTACATTCATCACTTTGACCGTGTCATTGTGATGGGCGCAGAGGCTCACGTCAACACCTGCTACAA
ACACTTCTATTACTTCGTCACAGAGATGAACCTCATAGACCGCAAGGAGCTGGAGCCCTTGAAAGAGATG
ACAACCAGGATGTGTCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202388 representing NM_178061
Red=Cloning site Green=Tags(s)

MSIALKQVFNKDKTFRPKRKFEPGTQRFELHKRAQASLNSGVDLRAAVQLPNGEDQNDWVAVHVVDFFNR
INLIYGTICEFCTERTCPVMSGGPKYEYRWQDDLKYKKPTALPAPQYMNLLMDWIEVQINNEDIFPTCVG
VPFPKNFLQICKKILCRLFRVFVHVYIHHFDRVIVMGAEAHVNTCYKHFYYFVTEMNLIDRKELEPLKEM
TTRMCH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178061
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178061.3
RefSeq Size 2382 bp
RefSeq ORF 651 bp
Locus ID 214944
UniProt ID Q8VE04
Cytogenetics 4 16.86 cM
Summary Modulates LATS1 expression in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178061" in other vectors (3)
SKU Description Size Price
MR202388 Mob3b (Myc-DDK-tagged) - Mouse MOB1, Mps One Binder kinase activator-like 2B (yeast) (Mobkl2b) 10 ug
$289.00 $300.00
MR202388L3 Lenti ORF clone of Mob3b (Myc-DDK-tagged) - Mouse MOB1, Mps One Binder kinase activator-like 2B (yeast) (Mobkl2b) 10 ug
$600.00
MR202388L4 Lenti ORF clone of Mob3b (mGFP-tagged) - Mouse MOB1, Mps One Binder kinase activator-like 2B (yeast) (Mobkl2b) 10 ug
$600.00

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