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Ccdc28b (NM_025455) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202023
Ccdc28b (tGFP-tagged) - Mouse coiled coil domain containing 28B (Ccdc28b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc28b
Synonyms 1110002E08Rik; 1810010N17Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202023 representing NM_025455
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGACAAAAAGAAGAAACGGAGTCCTAAGCCCTGCCTGACCCAACAAGCACAGGCCCCAGGCACAC
TGCGCAGAGTCCCTGTGCCCACCAGCCACAGTGGCTCCTTGGCTCTGGGACTCCCTCATTTGCCATCACC
TAAGCAGCGAGCCAAGTTCAAGAGAGCAGGCAAGGAGAAATGCCGACCGGTGCTGGCTGGAGGTGGAGGC
GGCTCCGCAGGCACACCCCTGCAACACTCCTTCCTGACAGAGGTGACAGATGTTTATGAGATGGAGGGAG
GACTGCTGAACTTGCTCAATGATTTTCATTCAGGCCGCCTGCAGGCCTTTGGGAAGGAATGTTCCTTCGA
GCAGCTGGAGCATGTTCGGGAGATGCAGGAGAAGTTGGCCAGACTGCACTTCAGCCTGGATGTGTGTGGG
GAGGAAGAGGACGAGGAAGAAGAGGAGGATGGGGTCACAGAAGGCTTGCCTGAGGAGCAGAAGAAGACAA
TGGCTGACCGCAACCTGGACCAGCTGCTTAGCAATCTGGAAGATCTTAGCAATTCCATACAGAAGCTACA
CTTGGCAGAGAATGCTGAGCCTGAGGACCAGCCAGCTGCG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202023 representing NM_025455
Red=Cloning site Green=Tags(s)

MEDKKKKRSPKPCLTQQAQAPGTLRRVPVPTSHSGSLALGLPHLPSPKQRAKFKRAGKEKCRPVLAGGGG
GSAGTPLQHSFLTEVTDVYEMEGGLLNLLNDFHSGRLQAFGKECSFEQLEHVREMQEKLARLHFSLDVCG
EEEDEEEEEDGVTEGLPEEQKKTMADRNLDQLLSNLEDLSNSIQKLHLAENAEPEDQPAA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025455
ORF Size 600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025455.1
RefSeq Size 856 bp
RefSeq ORF 603 bp
Locus ID 66264
UniProt ID Q8CEG5
Cytogenetics 4 D2.2
Summary Involved in ciliogenesis. Regulates cilia length through its interaction with MAPKAP1/SIN1 but independently of mTORC2 complex. Modulates mTORC2 complex assembly and function, possibly enhances AKT1 phosphorylation. Does not seem to modulate assembly and function of mTORC1 complex.UniProtKB/Swiss-Prot Function
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"NM_025455" in other vectors (4)
SKU Description Size Price
MC204500 Ccdc28b (untagged) - Mouse coiled coil domain containing 28B (Ccdc28b), (10ug) 10 ug
$300.00
MR202023 Ccdc28b (Myc-DDK-tagged) - Mouse coiled coil domain containing 28B (Ccdc28b) 10 ug
$289.00 $300.00
MR202023L3 Lenti ORF clone of Ccdc28b (Myc-DDK-tagged) - Mouse coiled coil domain containing 28B (Ccdc28b) 10 ug
$600.00
MR202023L4 Lenti ORF clone of Ccdc28b (mGFP-tagged) - Mouse coiled coil domain containing 28B (Ccdc28b) 10 ug
$600.00

Plasmid Map for MG202023

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG202023
Circular map for MG202023

file-alt Citations

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