Commd2 (NM_175095) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202019
Commd2 (tGFP-tagged) - Mouse COMM domain containing 2 (Commd2)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Commd2
Synonyms 1190017G07Rik; AI047739; D3Ertd176e; HSPC042
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202019 representing NM_175095
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGGATTTGTCCGAGGAGCACAAGGAGCACCTGGCCTTCCTGCCGCAAGTGGACACTGCAGTGG
TCGCCGAGTTCGGGAGGATCGCCGTGGAGTTCCTCCGACGGGGCTCAAACCCGAAGATCTACGAAGGCGC
CGCCAGAAAACTGAACGTGAGTAGTGACACTATCCAGCATGGTGTGGAAGGATTAACGTATCTCCTCACC
GAGAGCTCAAAGCTCATGATTTCTGAACTGGATTTCCAAGACTCTGTCTTTGTTCTGGGATTCTCTGAAG
AACTAAACAAATTATTGCTTCAGCTCTACCTGGACAACAGAAAGGAGATCAGAACTATTCTGAACGAGTT
AGCACCTCGCCTTCCCAGTTACCATAGTCTTGAGTGGCGGCTAGATGTACAGCTTGCAAGCAGAAGTCTC
CGGCAACAGATTAAGCCAGCAGTGACCATAAAGCTGCACCTCGATCAGAATGGCGACCACAGCACTCATT
TCTTGCAGACAGACCCAGCCACCCTGCTTCATTTGGTTCAGCAGCTGGAGCAGGCCTTAGAGGAAATGAA
AACAAACCACTGCAGGAGAGTAGTCCGCAGCATCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202019 representing NM_175095
Red=Cloning site Green=Tags(s)

MLLDLSEEHKEHLAFLPQVDTAVVAEFGRIAVEFLRRGSNPKIYEGAARKLNVSSDTIQHGVEGLTYLLT
ESSKLMISELDFQDSVFVLGFSEELNKLLLQLYLDNRKEIRTILNELAPRLPSYHSLEWRLDVQLASRSL
RQQIKPAVTIKLHLDQNGDHSTHFLQTDPATLLHLVQQLEQALEEMKTNHCRRVVRSIK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175095
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175095.3, NP_780304.2
RefSeq Size 2962 bp
RefSeq ORF 600 bp
Locus ID 52245
UniProt ID Q8BXC6
Cytogenetics 3 27.94 cM
Summary May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. May down-regulate activation of NF-kappa-B.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175095" in other vectors (4)
SKU Description Size Price
MC207492 Commd2 (untagged) - Mouse COMM domain containing 2 (Commd2), (10ug) 10 ug
$330.00
MR202019 Commd2 (Myc-DDK-tagged) - Mouse COMM domain containing 2 (Commd2) 10 ug
$289.00 $300.00
MR202019L3 Lenti ORF clone of Commd2 (Myc-DDK-tagged) - Mouse COMM domain containing 2 (Commd2) 10 ug
$600.00
MR202019L4 Lenti ORF clone of Commd2 (mGFP-tagged) - Mouse COMM domain containing 2 (Commd2) 10 ug
$600.00

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