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BC017643 (NM_144832) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201751
BC017643 (tGFP-tagged) - Mouse cDNA sequence BC017643 (BC017643)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $500.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol BC017643
Synonyms MGC28978; MGC37062; MGC37382
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201751 representing NM_144832
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATATGCAGGTGGAGACACGCACCAGCACCCGCCTCCATCTGAAGAGGGCTCCAGGCATCCGGTCTT
GGTCCCTGCTGGTCGGAATATTATCCACTGGCCTGGCTGCTGCCTATTACAGTGGAGATAGTCTGGGCTG
GAAGCTCTTCTATGTCACAGGCTGCCTATTTGTGGCTGTACAGAACTTGGAAGACTGGGAGGAGGCCATT
TTCAACAAGAACACTGGGAAGGTCATCTTAAAAACATTCAGCCTCTACAAGAAGCTGCTGACTCTTCTCA
GAGCGGGCCACGATCAAGTGGTGGTTCTGTTGAAAGACATCCAGGATGTGAATGTGGAGGAGGAAAAGGT
CCGTTACTTTGGGAAGGGCTACATGGTGGTGCTCCGGTTTGCAACAGGCTTCTCCCACCCTCTCACTCAG
AGTGCAGTCATGGGCCGACGCAGTGATGTGGAAGCCATTGCCAAACTCATCACTAGCTTCTTGGAGCTGC
ACCGCCTGGAGAGCCCCTCAGAACGGTCACAGAGCAGCGACAGTGAACCTGATGGCCCTGGTGGCCAGAG
T

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201751 representing NM_144832
Red=Cloning site Green=Tags(s)

MYMQVETRTSTRLHLKRAPGIRSWSLLVGILSTGLAAAYYSGDSLGWKLFYVTGCLFVAVQNLEDWEEAI
FNKNTGKVILKTFSLYKKLLTLLRAGHDQVVVLLKDIQDVNVEEEKVRYFGKGYMVVLRFATGFSHPLTQ
SAVMGRRSDVEAIAKLITSFLELHRLESPSERSQSSDSEPDGPGGQS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144832
ORF Size 561 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144832.2
RefSeq Size 2439 bp
RefSeq ORF 564 bp
Locus ID 217370
UniProt ID Q3TYS2
Cytogenetics 11 E2
Summary Necessary for a stable expression of the CYBA and CYBB subunits of the cytochrome b-245 hetrodimer. Controls the phagocyte respiratory burst and is essential for innate immunity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144832" in other vectors (4)
SKU Description Size Price
MC201263 BC017643 (untagged) - Mouse cDNA sequence BC017643 (BC017643), (10ug) 10 ug
$300.00
MR201751 BC017643 (Myc-DDK-tagged) - Mouse cDNA sequence BC017643 (BC017643) 10 ug
$289.00 $300.00
MR201751L3 Lenti ORF clone of BC017643 (Myc-DDK-tagged) - Mouse cDNA sequence BC017643 (BC017643) 10 ug
$600.00
MR201751L4 Lenti ORF clone of BC017643 (mGFP-tagged) - Mouse cDNA sequence BC017643 (BC017643) 10 ug
$600.00

Plasmid Map for MG201751

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG201751
Circular map for MG201751

file-alt Citations

Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity
Thomas, Clare, Sowerby et al
J. Exp. Med. (2017)
Cited by 73
AI Summary: OriGene’s MG201751 plasmid, containing the bc017643 coding sequence, was used as a template to amplify and study the Eros gene, enabling the researchers to investigate its essential role in innate immunity.
Snippet: In brief, bc017643 coding sequence was ampli- fied from a plasmid template (MG201751;Origene) using the primers listed below
Showing 1-1 of 1 papers.
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