Rap2b (NM_028712) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201667
Rap2b (tGFP-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rap2b
Synonyms 4021402C18Rik; AA408554
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201667 representing NM_028712
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGAGTACAAAGTGGTGGTACTGGGCTCGGGCGGCGTGGGCAAGTCCGCGCTCACCGTGCAGTTCG
TAACAGGTTCCTTCATCGAGAAGTACGACCCGACCATCGAGGACTTTTACCGCAAGGAGATCGAGGTGGA
CTCGTCGCCGTCGGTGCTGGAGATCTTGGACACCGCGGGCACGGAGCAGTTCGCCTCAATGCGGGACCTG
TACATCAAGAATGGCCAGGGCTTCATTCTCGTCTACAGCCTGGTCAACCAGCAGAGCTTCCAGGACATCA
AGCCCATGCGGGACCAGATCATCCGCGTGAAGCGGTACGAGCGCGTACCCATGATCCTGGTAGGCAACAA
GGTGGACTTGGAGGGTGAACGTGAGGTCTCCTATGGCGAGGGTAAGGCCCTGGCCGAGGAGTGGAGCTGC
CCCTTCATGGAGACATCGGCCAAAAACAAAGCCTCAGTGGATGAGCTATTCGCAGAGATCGTGAGGCAGA
TGAACTACGCGGCACAGCCCAACGGCGACGAGGGCTGCTGCTCGGCCTGCGTGATCCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201667 representing NM_028712
Red=Cloning site Green=Tags(s)

MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDL
YIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSYGEGKALAEEWSC
PFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSACVIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028712
ORF Size 549 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028712.4, NP_082988.1
RefSeq Size 4197 bp
RefSeq ORF 552 bp
Locus ID 74012
UniProt ID P61226
Cytogenetics 3 E1
Summary Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028712" in other vectors (4)
SKU Description Size Price
MC204890 Rap2b (untagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b), (10ug) 10 ug
$300.00
MR201667 Rap2b (Myc-DDK-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b) 10 ug
$289.00 $300.00
MR201667L3 Lenti ORF clone of Rap2b (Myc-DDK-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b) 10 ug
$600.00
MR201667L4 Lenti ORF clone of Rap2b (mGFP-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b) 10 ug
$600.00

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