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Sod2 (BC066063) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201642
Sod2 (tGFP-tagged) - Mouse superoxide dismutase 2, mitochondrial (cDNA clone MGC:90127 IMAGE:6849521)
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ORF Plasmid:
tGFP DDK
  $650.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sod2
Synonyms MnSOD, MGC6144
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC066063
ORF Size 546 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC066063
RefSeq Size 4089 bp
RefSeq ORF 548 bp
Locus ID 20656
Cytogenetics 17 8.75 cM
Summary Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.UniProtKB/Swiss-Prot Function
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"BC066063" in other vectors (5)
SKU Description Size Price
MR201642 Sod2 (Myc-DDK-tagged) - Mouse superoxide dismutase 2, mitochondrial (cDNA clone MGC:90127 IMAGE:6849521) 10 ug
$450.00
MR201642L1 Lenti ORF clone of Sod2 (Myc-DDK-tagged) - Mouse superoxide dismutase 2, mitochondrial (cDNA clone MGC:90127 IMAGE:6849521) 10 ug
$750.00
MR201642L2 Lenti ORF clone of Sod2 (mGFP-tagged) - Mouse superoxide dismutase 2, mitochondrial (cDNA clone MGC:90127 IMAGE:6849521) 10 ug
$750.00
MR201642L3 Lenti ORF clone of Sod2 (Myc-DDK-tagged) - Mouse superoxide dismutase 2, mitochondrial (cDNA clone MGC:90127 IMAGE:6849521) 10 ug
$750.00
MR201642L4 Lenti ORF clone of Sod2 (mGFP-tagged) - Mouse superoxide dismutase 2, mitochondrial (cDNA clone MGC:90127 IMAGE:6849521) 10 ug
$750.00

Plasmid Map for MG201642

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Cloning scheme for MG201642
Circular map for MG201642

file-alt Citations

Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue
Rosina, Ceci, Turchi et al
Cell Metab (2022) 34 (4), 533-548.e12 DOI: 10.1016/j.cmet.2022.02.016
Cited by 274
AI Summary: OriGene’s dhb (NM_024221) Mouse Tagged ORF Clone Lentiviral Particle was used in this study to manipulate gene expression in brown adipocytes, facilitating the investigation of mitochondrial dynamics and extracellular vesicle release during thermogenic stress.
Snippet: dhb (NM_024221) Mouse Tagged ORF Clone Lentiviral Particle OriGene Technologies
Showing 1-1 of 1 papers.
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