OriGene Logo

Lim2 (NM_177693) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG201478
Lim2 (tGFP-tagged) - Mouse lens intrinsic membrane protein 2 (Lim2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Lim2 or NM_177693"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lim2
Synonyms 19kDa; 4833403J20; MP19; To3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201478 representing NM_177693
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACAGCTTCATGGGTGGCGGCCTCTTCTGTGCCTGGGTGGGGACCATCCTGTTGGTGGTAGCCACAG
CAACTGACCACTGGATGCAGTACCGGCTGTCGGGGTCCTTCGCACACCAGGGCCTGTGGCGTTACTGCCT
GGGCAACAAGTGCTTCCTGCAGACCGAGAGCATCGCATATTGGAATGCCACCCGGGCTTTCATGATCCTG
TCTGCCCTGTGTGCCACCTCGGGCATCATCATGGGTGTCCTAGCCTTTGCGCAGCAATCCACCTTCACCC
GTCTCTCCAGGCCCTTCTCTGCCGGCATCATGTTTTTTGCCTCCACCCTTTTTGTCCTGTTGTCCTTGGC
TATTTACACTGGAGTCACCGTCAGTTTCCTCGGCCGCCGCTTTGGAGACTGGCGCTTTTCATGGTCTTAC
ATCCTGGGCTGGGTGGCCCTGCTCATGACCTTCTTTGCAGGAATTTTCTACATGTGTGCCTACCGGATGC
ATGAGTGCCGGCGCCTAGCCACCCCACGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201478 representing NM_177693
Red=Cloning site Green=Tags(s)

MYSFMGGGLFCAWVGTILLVVATATDHWMQYRLSGSFAHQGLWRYCLGNKCFLQTESIAYWNATRAFMIL
SALCATSGIIMGVLAFAQQSTFTRLSRPFSAGIMFFASTLFVLLSLAIYTGVTVSFLGRRFGDWRFSWSY
ILGWVALLMTFFAGIFYMCAYRMHECRRLATPR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177693
ORF Size 519 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177693.2, NP_808361.1
RefSeq Size 868 bp
RefSeq ORF 522 bp
Locus ID 233187
UniProt ID P56563
Cytogenetics 7 28.25 cM
Summary Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_177693" in other vectors (4)
SKU Description Size Price
MC207914 Lim2 (untagged) - Mouse lens intrinsic membrane protein 2 (Lim2), (10ug) 10 ug
$330.00
MR201478 Lim2 (Myc-DDK-tagged) - Mouse lens intrinsic membrane protein 2 (Lim2) 10 ug
$289.00 $300.00
MR201478L3 Lenti ORF clone of Lim2 (Myc-DDK-tagged) - Mouse lens intrinsic membrane protein 2 (Lim2) 10 ug
$600.00
MR201478L4 Lenti ORF clone of Lim2 (mGFP-tagged) - Mouse lens intrinsic membrane protein 2 (Lim2) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.