Txnl4b (NM_175646) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201042
Txnl4b (tGFP-tagged) - Mouse thioredoxin-like 4B (Txnl4b)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Txnl4b
Synonyms AI595343; D530025J19Rik; Dim2; DLP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201042 representing NM_175646
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTTCCTCTTGCCCAAACTGACCAGCAAGAAAGAAGTAGACCAGGCGATCAAAAGCACTGCGGAGA
AGGTGTTGGTTCTCAGGTTTGGGAGGGACGAGGACCCCGTCTGTCTGCAGCTGGATGATATTCTCTCAAA
AACTTCAGCCGACCTGAGTAAAATGGCTGCTATTTACCTGGTGGACGTGGACCACACGCCGGTTTACACA
CAGTATTTTGACATCAGTTACATTCCCTCTACCGTATTTTTCTTCAATGGGCAGCACATGAAGGTGGATT
ATGGGTCTCCGGATCACACTAAATTCGTAGGAAGCTTCAAAACCAAACAAGACTTCATAGATTTGATTGA
AGTCATCTACCGGGGAGCGATGAGGGGAAAACTTATTGTCCAGAGTCCTATTGATCCCAAGAACGTGCCC
AAATATGACCTCCTCTATCAAGACATT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201042 representing NM_175646
Red=Cloning site Green=Tags(s)

MSFLLPKLTSKKEVDQAIKSTAEKVLVLRFGRDEDPVCLQLDDILSKTSADLSKMAAIYLVDVDHTPVYT
QYFDISYIPSTVFFFNGQHMKVDYGSPDHTKFVGSFKTKQDFIDLIEVIYRGAMRGKLIVQSPIDPKNVP
KYDLLYQDI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175646
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175646.4
RefSeq Size 1761 bp
RefSeq ORF 450 bp
Locus ID 234723
UniProt ID Q8BUH1
Cytogenetics 8 D3
Summary Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175646" in other vectors (4)
SKU Description Size Price
MC207918 Txnl4b (untagged) - Mouse thioredoxin-like 4B (Txnl4b), (10ug) 10 ug
$165.00
MR201042 Txnl4b (Myc-DDK-tagged) - Mouse thioredoxin-like 4B (Txnl4b) 10 ug
$289.00
MR201042L3 Lenti ORF clone of Txnl4b (Myc-DDK-tagged) - Mouse thioredoxin-like 4B (Txnl4b) 10 ug
$450.00
MR201042L4 Lenti ORF clone of Txnl4b (mGFP-tagged) - Mouse thioredoxin-like 4B (Txnl4b) 10 ug
$450.00

Plasmid Map for MG201042

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Cloning scheme for MG201042

Circular map for MG201042

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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