Ifitm2 (NM_030694) Mouse Tagged ORF Clone
- TrueORF®
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ifitm2 |
| Synonyms | DSPA2c; fragilis3; Ifitm3l; mil-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_030694 |
| ORF Size | 432 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_030694.1, NP_109619.1 |
| RefSeq Size | 646 bp |
| RefSeq ORF | 435 bp |
| Locus ID | 80876 |
| UniProt ID | Q99J93 |
| Cytogenetics | 7 F5 |
| Summary | IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV) and West Nile virus (WNV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_030694" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC202748 | Ifitm2 (untagged) - Mouse interferon induced transmembrane protein 2 (Ifitm2), (10ug) | 10 ug |
$150.00
|
|
| MR200956 | Ifitm2 (Myc-DDK-tagged) - Mouse interferon induced transmembrane protein 2 (Ifitm2) | 10 ug |
$289.00
|
|
| MR200956L3 | Lenti ORF clone of Ifitm2 (Myc-DDK-tagged) - Mouse interferon induced transmembrane protein 2 (Ifitm2) | 10 ug |
$450.00
|
|
| MR200956L4 | Lenti ORF clone of Ifitm2 (mGFP-tagged) - Mouse interferon induced transmembrane protein 2 (Ifitm2) | 10 ug |
$450.00
|
|
Plasmid Map for MG200956
Download sequence for
Cloning scheme for MG200956
Circular map for MG200956
Citations
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