Phlda2 (NM_009434) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200950
Phlda2 (tGFP-tagged) - Mouse pleckstrin homology-like domain, family A, member 2 (Phlda2)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Phlda2
Synonyms Ipl; Tss; Tssc3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200950 representing NM_009434
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGAAAATCGTGATGTCTTCGAAAACCGTGAAGACCTCCGACGAGATCCTTTGCGAGGGCGAGC
TGGAGAAGCGAAGCGACAGCCTGTTCCAGGTATGGAAGAAGAAGCGCTGCGTGCTCACCGCCGACCGCCT
GCGCCTCTTCTCCGGGAAAACCAGCCCCGCCAAGGAGCTGTTTTTCCACTCCATCCTCAAGGTGGACTGC
GTGGAGCACACCTCTAAGTACGTGTACTTCACCATCGTCACCAACTATTACAAGGAGATCGACTTCCGCT
GCACGGTGGAGAGCTGCTGGAACGCGGCCATCACCATGGCGCTGATCGACTTCCAGAACCGTCGGGCGCT
GCAAGACTTTCCCCGCTACCGGTATCAGCGCTCTGAGTCTGAAATGCCTTCCGAGCCGGGAGAGCAGAGC
GCTCTGGGTCCG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200950 representing NM_009434
Red=Cloning site Green=Tags(s)

MASKIVMSSKTVKTSDEILCEGELEKRSDSLFQVWKKKRCVLTADRLRLFSGKTSPAKELFFHSILKVDC
VEHTSKYVYFTIVTNYYKEIDFRCTVESCWNAAITMALIDFQNRRALQDFPRYRYQRSESEMPSEPGEQS
ALGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009434
ORF Size 432 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009434.2, NP_033460.1
RefSeq Size 737 bp
RefSeq ORF 435 bp
Locus ID 22113
UniProt ID O08969
Cytogenetics 7 88.23 cM
Summary This gene is one of several genes in the imprinted gene domain on chromosome 7. Studies using knockout mice have shown that the product of this gene regulates placental growth. Transcripts from this gene are most abundant in placenta and yolk sac, and are almost entirely transcribed from the maternal allele. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_009434" in other vectors (4)
SKU Description Size Price
MC207432 Phlda2 (untagged) - Mouse pleckstrin homology-like domain, family A, member 2 (Phlda2), (10ug) 10 ug
$165.00
MR200950 Phlda2 (Myc-DDK-tagged) - Mouse pleckstrin homology-like domain, family A, member 2 (Phlda2) 10 ug
$289.00
MR200950L3 Lenti ORF clone of Phlda2 (Myc-DDK-tagged) - Mouse pleckstrin homology-like domain, family A, member 2 (Phlda2) 10 ug
$450.00
MR200950L4 Lenti ORF clone of Phlda2 (mGFP-tagged) - Mouse pleckstrin homology-like domain, family A, member 2 (Phlda2) 10 ug
$450.00

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