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Sprr1a (NM_009264) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200942
Sprr1a (tGFP-tagged) - Mouse small proline-rich protein 1A (Sprr1a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $425.00
In Stock*
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Other products for "Sprr1a or NM_009264"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sprr1a
Synonyms AI528815; mSPRR1A; SPR1a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200942 representing NM_009264
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTCCCACCAGCAGAAGCAGCCCTGCACTGTACCTCCTCAGCTGCACCAGCAGCAGGTGAAGCAGC
CTTGCCAGCCACCACCCCAGGAACCTTGTGCCCCCAAAACCAAGGATCCCTGCCACCCTGTTCCTGAGCC
CTGCAACCCCAAGGGGCCAGAGCCCTGCCACCCCAAGGCACCCGAGCCCTGCCACCCCAAGGCACCTGAG
CCCTGCAACCCCAAGGTGCCAGAGCCCTGCCAGCCTAAGGTGCCAGAGCCCTGCCAGCCTAAGGTGCCAG
AGCCCTGCAACCCCAAGGTGCCAGAGCCCTGCCAACCTAAGGCACCAGAGCCTTGCCACCCCAAGGCGCC
TGAGCCCTGCCACCCTGTTGTTCCCGAGCCCTGCCCCTCAACTGTCACTCCATCACCATACCAGCAGAAG
ACAAAGCAGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200942 representing NM_009264
Red=Cloning site Green=Tags(s)

MSSHQQKQPCTVPPQLHQQQVKQPCQPPPQEPCAPKTKDPCHPVPEPCNPKGPEPCHPKAPEPCHPKAPE
PCNPKVPEPCQPKVPEPCQPKVPEPCNPKVPEPCQPKAPEPCHPKAPEPCHPVVPEPCPSTVTPSPYQQK
TKQK

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009264
ORF Size 432 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009264.2, NP_033290.1
RefSeq Size 807 bp
RefSeq ORF 435 bp
Locus ID 20753
UniProt ID Q62266
Cytogenetics 3 40.14 cM
Summary Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. May participate widely in the construction of cell envelopes in cornifying epithelia characterized by either increased thickness or a requirement for extreme flexibility.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009264" in other vectors (4)
SKU Description Size Price
MC201375 Sprr1a (untagged) - Mouse small proline-rich protein 1A (Sprr1a), (10ug) 10 ug
$150.00
MR200942 Sprr1a (Myc-DDK-tagged) - Mouse small proline-rich protein 1A (Sprr1a) 10 ug
$225.00
MR200942L3 Lenti ORF clone of Sprr1a (Myc-DDK-tagged) - Mouse small proline-rich protein 1A (Sprr1a) 10 ug
$525.00
MR200942L4 Lenti ORF clone of Sprr1a (mGFP-tagged) - Mouse small proline-rich protein 1A (Sprr1a) 10 ug
$525.00

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