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Cdkn2b (NM_007670) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200733
Cdkn2b (tGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (Cdkn2b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $489.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdkn2b
Synonyms AV083695; INK4b; MTS2; p15; p15(INK4b); p15INK4b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200733 representing NM_007670
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGGCGGCAGCAGTGACGCGGGCCTGGCCACCGCCGCGGCGCGGGGGCAAGTGGAGACGGTGCGGC
AGCTCCTGGAAGCCGGCGCAGATCCCAACGCCCTGAACCGCTTCGGGAGGCGCCCAATCCAGGTCATGAT
GATGGGCAGCGCCCAGGTGGCAGAGCTGCTGCTGCTCCACGGAGCAGAACCCAACTGCGCCGACCCTGCC
ACCCTTACCAGACCTGTGCACGACGCAGCTCGGGAAGGCTTCCTGGACACGCTTGTCGTGCTGCACCGGG
CAGGGGCGCGGCTGGATGTGTGTGACGCCTGGGGCCGCCTGCCGGTAGACTTAGCTGAAGAGCAGGGCCA
CCGTGACATTGCGAGGTATCTGCACGCTGCCACTGGAGAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200733 representing NM_007670
Red=Cloning site Green=Tags(s)

MLGGSSDAGLATAAARGQVETVRQLLEAGADPNALNRFGRRPIQVMMMGSAQVAELLLLHGAEPNCADPA
TLTRPVHDAAREGFLDTLVVLHRAGARLDVCDAWGRLPVDLAEEQGHRDIARYLHAATGD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007670
ORF Size 390 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007670.2
RefSeq Size 1284 bp
RefSeq ORF 393 bp
Locus ID 12579
UniProt ID P55271
Cytogenetics 4 42.15 cM
Summary Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest (By similarity).UniProtKB/Swiss-Prot Function
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"NM_007670" in other vectors (4)
SKU Description Size Price
MC207249 Cdkn2b (untagged) - Mouse cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (Cdkn2b), (10ug) 10 ug
$165.00
MR200733 Cdkn2b (Myc-DDK-tagged) - Mouse cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (Cdkn2b) 10 ug
$289.00
MR200733L3 Lenti ORF clone of Cdkn2b (Myc-DDK-tagged) - Mouse cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (Cdkn2b) 10 ug
$450.00
MR200733L4 Lenti ORF clone of Cdkn2b (mGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (Cdkn2b) 10 ug
$450.00

Plasmid Map for MG200733

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG200733
Circular map for MG200733

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