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Gpha2 (NM_130453) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200702
Gpha2 (tGFP-tagged) - Mouse glycoprotein hormone alpha 2 (Gpha2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpha2
Synonyms GPA2; zsig51
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200702 representing NM_130453
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCATGGCACCACGAGTCTTGCTCCTTTGCCTGCTGGGCCTGGCAGTCACTGAAGGGCATAGCCCAG
AGACAGCCATCCCAGGCTGCCACTTGCACCCCTTCAATGTGACGGTGCGCAGTGATCGCCTCGGCACTTG
CCAGGGCTCCCACGTGGCACAGGCCTGTGTAGGACACTGTGAGTCTAGTGCTTTCCCTTCCCGGTACTCT
GTGCTGGTGGCCAGTGGCTATCGGCACAACATCACCTCTTCCTCCCAGTGCTGCACCATCAGCAGCCTCA
GAAAGGTGAGGGTGTGGCTGCAGTGCGTGGGGAACCAGCGTGGGGAGCTTGAGATCTTTACTGCAAGGGC
CTGCCAGTGTGATATGTGCCGTTTCTCCCGCTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200702 representing NM_130453
Red=Cloning site Green=Tags(s)

MPMAPRVLLLCLLGLAVTEGHSPETAIPGCHLHPFNVTVRSDRLGTCQGSHVAQACVGHCESSAFPSRYS
VLVASGYRHNITSSSQCCTISSLRKVRVWLQCVGNQRGELEIFTARACQCDMCRFSRY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130453
ORF Size 384 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130453.3, NP_569720.1
RefSeq Size 626 bp
RefSeq ORF 387 bp
Locus ID 170458
UniProt ID Q925Q5
Cytogenetics 19 A
Summary Functions as a heterodimeric glycoprotein hormone with GPHB5 able to bind and activate the thyroid-stimulating hormone receptor (TSHR), leading to increased cAMP production. Plays a central role in controlling thyroid cell metabolism.UniProtKB/Swiss-Prot Function
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"NM_130453" in other vectors (4)
SKU Description Size Price
MC207853 Gpha2 (untagged) - Mouse glycoprotein hormone alpha 2 (Gpha2), (10ug) 10 ug
$165.00
MR200702 Gpha2 (Myc-DDK-tagged) - Mouse glycoprotein hormone alpha 2 (Gpha2) 10 ug
$289.00
MR200702L3 Lenti ORF clone of Gpha2 (Myc-DDK-tagged) - Mouse glycoprotein hormone alpha 2 (Gpha2) 10 ug
$450.00
MR200702L4 Lenti ORF clone of Gpha2 (mGFP-tagged) - Mouse glycoprotein hormone alpha 2 (Gpha2) 10 ug
$450.00

Plasmid Map for MG200702

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG200702
Circular map for MG200702

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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