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Med11 (NM_025397) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200529
Med11 (tGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) (Med11)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Med11
Synonyms 1110030J09Rik; AI465144; AW545069
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200529 representing NM_025397
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACCTACAGTCTGGCGAACGAGCGGCTGCGCGCCCTGGAGGATATCGAGCGCGAAATCGGCGCCA
TCCTCCAGAACGCAGGAACGGCGATCCTGGAACTGTCCAAGGAAAAGACTAACGAGCGGCTCCTCGACCG
GCAGGCGGCAGCCTTCACCACTTCTGTGCAGCACGTGGAGGCAGAGCTATCCGCTCAAATCCGCTACCTC
ACTCAGGTGGCCACAGGGCAGCCGCATGAGGGCTCCAGCTACTCTTCCAGGAAGGACTGTCAAATGGCTC
TAAAGCGAGTAGACTACGCCCGCCTCAAGATTAGTGATGTGGCGAGAACCTGTGAGCAGATGCTGGAGAA
C

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200529 representing NM_025397
Red=Cloning site Green=Tags(s)

MATYSLANERLRALEDIEREIGAILQNAGTAILELSKEKTNERLLDRQAAAFTTSVQHVEAELSAQIRYL
TQVATGQPHEGSSYSSRKDCQMALKRVDYARLKISDVARTCEQMLEN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025397
ORF Size 351 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025397.2, NP_079673.2
RefSeq Size 903 bp
RefSeq ORF 354 bp
Locus ID 66172
UniProt ID Q9D8C6
Cytogenetics 11 B3
Summary Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_025397" in other vectors (4)
SKU Description Size Price
MC202233 Med11 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) (Med11), (10ug) 10 ug
$150.00
MR200529 Med11 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) (Med11) 10 ug
$289.00
MR200529L3 Lenti ORF clone of Med11 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) (Med11) 10 ug
$450.00
MR200529L4 Lenti ORF clone of Med11 (mGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) (Med11) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.