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Guca2a (NM_008190) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200499
Guca2a (tGFP-tagged) - Mouse guanylate cyclase activator 2a (guanylin) (Guca2a)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Guca2a
Synonyms Guca2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200499 representing NM_008190
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGCCTGTGTGCTCTCTGTGCTGTGCCTCCTGGGTGCCTTGGCTGTCCTGGTAGAAGGGGTCACTG
TGCAGGATGGAGACCTCTCCTTTCCTCTGGAGTCAGTGAAAAAACTTAAGGGTCTCCGCGAGGTACAGGA
GCCCAGACTGGTGAGTCACAAGAAGTTTGCTCCCCGGCTTCTTCAGCCCGTGGCACCACAGCTATGTAGT
AGCCACTCTGCACTTCCAGAAGCACTGAGGCCGGTCTGCGAGAAACCCAACGCCGAGGAGATCCTGCAGA
GGCTAGAGGCCATTGCTCAGGACCCAAACACATGTGAGATCTGTGCCTACGCTGCCTGTACGGGATGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200499 representing NM_008190
Red=Cloning site Green=Tags(s)

MNACVLSVLCLLGALAVLVEGVTVQDGDLSFPLESVKKLKGLREVQEPRLVSHKKFAPRLLQPVAPQLCS
SHSALPEALRPVCEKPNAEEILQRLEAIAQDPNTCEICAYAACTGC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008190
ORF Size 348 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008190.1, NP_032216.1
RefSeq Size 560 bp
RefSeq ORF 351 bp
Locus ID 14915
UniProt ID P33680
Cytogenetics 4 55.47 cM
Summary Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008190" in other vectors (4)
SKU Description Size Price
MC200878 Guca2a (untagged) - Mouse guanylate cyclase activator 2a (guanylin) (Guca2a), (10ug) 10 ug
$150.00
MR200499 Guca2a (Myc-DDK-tagged) - Mouse guanylate cyclase activator 2a (guanylin) (Guca2a) 10 ug
$289.00
MR200499L3 Lenti ORF clone of Guca2a (Myc-DDK-tagged) - Mouse guanylate cyclase activator 2a (guanylin) (Guca2a) 10 ug
$450.00
MR200499L4 Lenti ORF clone of Guca2a (mGFP-tagged) - Mouse guanylate cyclase activator 2a (guanylin) (Guca2a) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.