Retnla (BC029248) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200435
Retnla (tGFP-tagged) - Mouse resistin like alpha (cDNA clone MGC:35890 IMAGE:4189285)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Retnla
Synonyms HIMF, RELMalpha, Xcp2, Fizz1, RELMa, RELM-alpha
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200435 representing BC029248
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACTACAACTTGTTCCCTTCTCATCTGCATCTCCCTGCTCCAGCTGATGGTCCCAGTGAATACTG
ATGAGACCATAGAGATTATCGTGGAGAATAAGGTCAAGGAACTTCTTGCCAATCCAGCTAACTATCCCTC
CACTGTAACGAAGACTCTCTCTTGCACTAGTGTCAAGACTATGAACAGATGGGCCTCCTGCCCTGCTGGG
ATGACTGCTACTGGGTGTGCTTGTGGCTTTGCCTGTGGATCTTGGGAGATCCAGAGTGGAGATACTTGCA
ACTGCCTGTGCTTACTCGTTGACTGGACCACTGCCCGCTGCTGCCAACTGTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200435 representing BC029248
Red=Cloning site Green=Tags(s)

MKTTTCSLLICISLLQLMVPVNTDETIEIIVENKVKELLANPANYPSTVTKTLSCTSVKTMNRWASCPAG
MTATGCACGFACGSWEIQSGDTCNCLCLLVDWTTARCCQLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC029248
ORF Size 333 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC029248, AAH29248
RefSeq Size 554 bp
RefSeq ORF 335 bp
Locus ID 57262
Cytogenetics 16 30.75 cM
Summary Probable hormone. Plays a role in pulmonary vascular remodeling.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC029248" in other vectors (4)
SKU Description Size Price
MC206882 Retnla (untagged) - Mouse resistin like alpha (cDNA clone MGC:35890 IMAGE:4189285), (10ug) 10 ug
$165.00
MR200435 Retnla (Myc-DDK-tagged) - Mouse resistin like alpha (cDNA clone MGC:35890 IMAGE:4189285) 10 ug
$289.00
MR200435L3 Lenti ORF clone of Retnla (Myc-DDK-tagged) - Mouse resistin like alpha (cDNA clone MGC:35890 IMAGE:4189285) 10 ug
$450.00
MR200435L4 Lenti ORF clone of Retnla (mGFP-tagged) - Mouse resistin like alpha (cDNA clone MGC:35890 IMAGE:4189285) 10 ug
$450.00

Plasmid Map for MG200435

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Cloning scheme for MG200435

Circular map for MG200435

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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