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Ccl19 (BC051472) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200395
Ccl19 (tGFP-tagged) - Mouse chemokine (C-C motif) ligand 19 (cDNA clone MGC:62903 IMAGE:1349213)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccl19
Synonyms ELC, MIP3B, exodus-3, CKb11
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200395 representing BC051472
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCCGTGTGACCCCACTCCTGGCCTTCAGCCTGCTGGTTCTCTGGACCTTCCCAGCCCCAACTC
TGGGGGGTGCTAATGATGCGGAAGACTGCTGCCTGTCTGTGACCCAGCGCCCCATCCCTGGGAACATCGT
GAAAGCCTTCCGCTACCTTCTTAATGAAGATGGCTGCAGGGTGCCTGCTGTTGTGTTCACCACACTAAGG
GGCTATCAGCTCTGTGCACCTCCTGACCAGCCCTGGGTGGATCGCATCATCCGAAGACTGAAGAAGTCTT
CTGCCAAGAACAAAGGCAACAGCACCAGAAGGAGCCCTGTGTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200395 representing BC051472
Red=Cloning site Green=Tags(s)

MAPRVTPLLAFSLLVLWTFPAPTLGGANDAEDCCLSVTQRPIPGNIVKAFRYLLNEDGCRVPAVVFTTLR
GYQLCAPPDQPWVDRIIRRLKKSSAKNKGNSTRRSPVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC051472
ORF Size 324 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC051472, AAH51472
RefSeq Size 734 bp
RefSeq ORF 326 bp
Locus ID 24047
Cytogenetics 4 A5
Summary Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.UniProtKB/Swiss-Prot Function
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"BC051472" in other vectors (5)
SKU Description Size Price
MR200395 Ccl19 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 19 (cDNA clone MGC:62903 IMAGE:1349213) 10 ug
$289.00
MR200395L1 Lenti ORF clone of Ccl19 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 19 (cDNA clone MGC:62903 IMAGE:1349213) 10 ug
$450.00
MR200395L2 Lenti ORF clone of Ccl19 (mGFP-tagged) - Mouse chemokine (C-C motif) ligand 19 (cDNA clone MGC:62903 IMAGE:1349213) 10 ug
$450.00
MR200395L3 Lenti ORF clone of Ccl19 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 19 (cDNA clone MGC:62903 IMAGE:1349213) 10 ug
$450.00
MR200395L4 Lenti ORF clone of Ccl19 (mGFP-tagged) - Mouse chemokine (C-C motif) ligand 19 (cDNA clone MGC:62903 IMAGE:1349213) 10 ug
$450.00

Plasmid Map for MG200395

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MG200395
Circular map for MG200395

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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