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Ncmap (NM_145555) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200304
Ncmap (tGFP-tagged) - Mouse RIKEN cDNA A330049M08 gene (A330049M08Rik)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ncmap
Synonyms A330049M08Rik; AA545175; Mp11
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200304 representing NM_145555
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACAGCCACCACTTTGGGGGACGCCGTCTTCTCGCTGAACATGACCAGGGGAGAGGACGCCCTGT
ATAAGAGCTCTGGAGCCATCGTGGCTGCCATTGTGGTGGTTGTCATCATCATTGTCACCTTGGTGCTGAT
CCTGCTGAAGATGTACAACAGGAGAATGAGGACCAGGCGGGAGCTGGAGCCCAAGAGCCCAAAGCCACCT
GTCCCTCCTGCCCTGGACCCCAGCAGCAATGGCAGCCAGCAGCCTGCTACTGTGACCTTCGACCCTGCCA
ATGTCCATGTGGAGACTCGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200304 representing NM_145555
Red=Cloning site Green=Tags(s)

MTTATTLGDAVFSLNMTRGEDALYKSSGAIVAAIVVVVIIIVTLVLILLKMYNRRMRTRRELEPKSPKPP
VPPALDPSSNGSQQPATVTFDPANVHVETR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145555
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145555.2, NP_663530.1
RefSeq Size 1682 bp
RefSeq ORF 303 bp
Locus ID 230822
UniProt ID Q99JS0
Cytogenetics 4 D3
Summary Plays a role in myelin formation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145555" in other vectors (5)
SKU Description Size Price
MC203754 Ncmap (untagged) - Mouse RIKEN cDNA A330049M08 gene (A330049M08Rik), transcript variant 1, (10ug) 10 ug
$150.00
MG215448 Ncmap (tGFP-tagged) - Mouse RIKEN cDNA A330049M08 gene (A330049M08Rik) transcript variant 1, (10ug) 10 ug
$350.00
MR215448 Ncmap (Myc-DDK-tagged) - Mouse RIKEN cDNA A330049M08 gene (A330049M08Rik), transcript variant 1 10 ug
$289.00
MR215448L3 Lenti ORF clone of Ncmap (Myc-DDK-tagged) - Mouse RIKEN cDNA A330049M08 gene (A330049M08Rik), transcript variant 1 10 ug
$450.00
MR215448L4 Lenti ORF clone of Ncmap (mGFP-tagged) - Mouse RIKEN cDNA A330049M08 gene (A330049M08Rik), transcript variant 1 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.