Cox6b2 (NM_183406) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200215
Cox6b2 (tGFP-tagged) - Mouse cytochrome c oxidase subunit VIb polypeptide 2 (Cox6b2)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cox6b2
Synonyms 1700067P11Rik; BC048670; COXVIB2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200215 representing NM_183406
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGGTGTTCAAGCCCAGAAGCCCCCTCCGGGCCAATGGACAACGCCGCCCTTTGATCCGCGCTTCC
CTAACCAGAACCAGACGCGTAACTGCTACCAGAATTTTCTGGACTACCACCGGTGTGTGAAGACCATGAA
TCGCCGCGGAAAGAGCACACAACCCTGCGAGTACTATTTCCGTGTATTCCATTCACTGTGTCCCATCAGC
TGGGTGCAGCGCTGGAATGAGCAGATCAAGCAGGGAACTTTCCCGGGCAAAATA


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200215 representing NM_183406
Red=Cloning site Green=Tags(s)

MLGVQAQKPPPGQWTTPPFDPRFPNQNQTRNCYQNFLDYHRCVKTMNRRGKSTQPCEYYFRVFHSLCPIS
WVQRWNEQIKQGTFPGKI

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_183406
ORF Size 264 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183406.3
RefSeq Size 491 bp
RefSeq ORF 267 bp
Locus ID 333182
UniProt ID Q80ZN9
Cytogenetics 7 A1
Summary This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. This protein may be one of the heme-binding subunits of the oxidase (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_183406" in other vectors (3)
SKU Description Size Price
MR200215 Cox6b2 (Myc-DDK-tagged) - Mouse cytochrome c oxidase subunit VIb polypeptide 2 (Cox6b2), transcript variant 2 10 ug
$289.00
MR200215L3 Lenti ORF clone of Cox6b2 (Myc-DDK-tagged) - Mouse cytochrome c oxidase subunit VIb polypeptide 2 (Cox6b2), transcript variant 2 10 ug
$450.00
MR200215L4 Lenti ORF clone of Cox6b2 (mGFP-tagged) - Mouse cytochrome c oxidase subunit VIb polypeptide 2 (Cox6b2), transcript variant 2 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.