Gng3 (NM_010316) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200101
Gng3 (tGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 3 subunit (Gng3)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gng3
Synonyms 83
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200101 representing NM_010316
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGGGGAGACCCCCGTTAACAGCACTATGAGTATTGGTCAAGCACGCAAGATGGTGGAACAGCTTA
AGATTGAGGCCAGCTTGTGCCGGATAAAGGTGTCCAAGGCAGCAGCAGACCTGATGACATACTGTGATGC
CCACGCTTGTGAGGACCCCCTCATCACTCCTGTGCCCACTTCCGAGAACCCCTTCCGGGAGAAGAAGTTC
TTCTGTGCGCTCCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200101 representing NM_010316
Red=Cloning site Green=Tags(s)

MKGETPVNSTMSIGQARKMVEQLKIEASLCRIKVSKAAADLMTYCDAHACEDPLITPVPTSENPFREKKF
FCALL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010316
ORF Size 225 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010316.3, NP_034446.1
RefSeq Size 1699 bp
RefSeq ORF 228 bp
Locus ID 14704
UniProt ID P63216
Cytogenetics 19 5.76 cM
Summary Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_010316" in other vectors (4)
SKU Description Size Price
MC201646 Gng3 (untagged) - Mouse guanine nucleotide binding protein (G protein), gamma 3 (Gng3), (10ug) 10 ug
$150.00
MR200101 Gng3 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 3 (Gng3) 10 ug
$289.00
MR200101L3 Lenti ORF clone of Gng3 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 3 (Gng3) 10 ug
$450.00
MR200101L4 Lenti ORF clone of Gng3 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 3 (Gng3) 10 ug
$450.00

Plasmid Map for MG200101

Download sequence for

Cloning scheme for MG200101

Circular map for MG200101

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products