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Pcp4 (NM_008791) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200038
Pcp4 (tGFP-tagged) - Mouse Purkinje cell protein 4 (Pcp4)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcp4
Synonyms P16Rimb19; Pcp-4; Pep19
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200038 representing NM_008791
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAGAGACAAAGTGCCGGAGCGACCAACGGAAAAGACAAGACGTCAGGAGATAATGATGGGCAGA
AGAAAGTCCAAGAAGAATTTGATATCGACATGGATGCACCAGAGACAGAGCGTGCAGCTGTGGCCATTCA
GTCTCAGTTCAGAAAATTCCAGAAGAAAAAGGCAGGATCACAGTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200038 representing NM_008791
Red=Cloning site Green=Tags(s)

MSERQSAGATNGKDKTSGDNDGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQKKKAGSQS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008791
ORF Size 186 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008791.3
RefSeq Size 669 bp
RefSeq ORF 189 bp
Locus ID 18546
UniProt ID P63054
Cytogenetics 16 56.97 cM
Summary Functions as a modulator of calcium-binding by calmodulin. Thereby, regulates calmodulin activity and the different processes it controls. For instance, may play a role in neuronal differentiation through activation of calmodulin-dependent kinase signaling pathways.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008791" in other vectors (4)
SKU Description Size Price
MC209153 Pcp4 (untagged) - Mouse Purkinje cell protein 4 (Pcp4), (10ug) 10 ug
$165.00
MR200038 Pcp4 (Myc-DDK-tagged) - Mouse Purkinje cell protein 4 (Pcp4) 10 ug
$289.00
MR200038L3 Lenti ORF clone of Pcp4 (Myc-DDK-tagged) - Mouse Purkinje cell protein 4 (Pcp4) 10 ug
$450.00
MR200038L4 Lenti ORF clone of Pcp4 (mGFP-tagged) - Mouse Purkinje cell protein 4 (Pcp4) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.