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Cdc42bpa (NM_001033285) Mouse Untagged Clone

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SKU
MC224883
Cdc42bpa (untagged) - Mouse CDC42 binding protein kinase alpha (Cdc42bpa), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,602.00
7 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cdc42bpa
Synonyms A930014J19Rik; DMPK-like
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224883 representing NM_001033285
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGAGAAGTGCGTTTGAGGCAGTTGGAGCAGTTTATTTTAGATGGGCCAGCTCAGACCAATGGGC
AGTGCTTCAGTGTGGAGACGCTACTGGACATCCTCATCTGTCTTTACGATGAATGCAATAATTCTCCATT
GAGAAGAGAGAAGAACATTCTTGAATATCTAGAATGGGCTAAACCATTTACTTCTAAAGTGAAGCAAATG
CGATTACATAGAGAAGACTTTGAAATATTAAAGGTGATTGGTCGAGGAGCTTTTGGGGAGGTTGCTGTAG
TTAAACTAAAAAATGCAGATAAAGTATTCGCCATGAAAATTCTGAACAAGTGGGAAATGCTGAAAAGAGC
TGAGACAGCGTGCTTTCGTGAAGAGAGGGATGTGTTGGTAAATGGAGACAGTAAGTGGATAACCACTCTG
CACTATGCTTTCCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTACTTA
CTCTGCTCAGCAAATTTGAAGATAGATTGCCTGAAGAGATGGCTCGGTTTTACTTGGCTGAGATGGTGAT
AGCCATTGACTCAGTTCACCAGCTCCACTATGTTCACAGAGACATCAAACCTGACAATATATTGATGGAT
ATGAATGGACATATTCGTTTAGCAGATTTTGGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTCCAGT
CCTCGGTGGCAGTTGGAACTCCAGATTATATTTCTCCGGAAATCCTTCAGGCTATGGAAGACGGAAAAGG
CAGATATGGACCAGAGTGTGACTGGTGGTCCTTGGGGGTCTGTATGTATGAGATGCTTTACGGAGAAACA
CCATTCTATGCAGAGTCTCTGGTGGAGACATATGGAAAAATTATGAATCACAAAGAGCGGTTTCAGTTTC
CAGCCCAAGTGACAGACGTGTCCGAAAATGCTAAAGATCTTATTCGAAGACTCATTTGTAGTAGAGAACA
TCGACTTGGCCAAAATGGAATAGAAGACTTTAAGAAGCATCCATTCTTCAGTGGAATTGATTGGGATAAC
ATTCGGAATTGTGAAGCACCTTACATCCCAGAAGTTAGTAGCCCGACAGATACGTCAAATTTTGATGTGG
ATGACGACTGTTTAAAAAATTCTGAAACAATGCCCCCACCAACACATACTGCATTTTCTGGCCATCATCT
ACCATTTGTTGGTTTTACATATACTAGTAGCTGTGTTCTTTCTGATCGGAGCTGTTTAAGAGTCACAGCA
GGCCCCACCTCATTGGACCTTGATGTCAGTGTCCAGAGGACTCTAGACAATAACTTAGCAACTGAGGCTT
ATGAAAGGAGAATTAAGCGGCTTGAGCAGGAAAAGCTTGAACTGACTAGAAAACTTCAAGAGTCAACACA
GACTGTTCAAGCTCTGCAATATTCAACTGTTGATGGTCCACTAACGGCAAGCAAAGATTTAGAAATTAAA
AGTCTGAAAGAAGAGATTGAAAAACTAAGGAAACAAGTAGCAGAAGTGAATCATTTGGAACAACAACTTG
AAGAAGCTAATTCTGTGAGACGAGAACTAGATGATGCTTTCAGGCAAATCAAAGCTTCTGAAAAACAAAT
CAAAACTTTGCAGCAGGAAAGAGAAGAACTAAATAAGGAACTAGTCCAGGCTAGTGAGCGATTAAAAAAC
CAATCCAAAGAGCTGAAAGATGCACACTGTCAGAGGAAACTGGCCATGCAGGAATTCATGGAGATCAATG
AGCGACTAACAGAATTGCACACCCAAAAGCAGAAACTTGCTCGCCATGTCCGAGATAAGGAAGAAGAGGT
GGACCTGGTGATGCAAAAAGCTGAGAGCTTAAGGCAGGAGCTGCGCAGAGCAGAAAGAGCCAAGAAAGAG
CTGGAAGTTCACACCGAAGCTCTAATTGCTGAAGCATCAAAGGACAAGAAGCTCCGAGAGCAGAGCGAAC
ATTATTCTAAGCAACTGGAAAACGAGTTGGAGGGCCTGAAGCAAAAACAAATTAGTTACTCACCAGGAAT
ATGCAGCATAGAACATCAGCAAGAGATAACTAAACTGAAAACTGACTTGGAAAAGAAAAGTATCTTTTAT
GAAGAAGAAATTTCTAAAAGAGAAGGAATTCACGCAAGTGAGATTAAAAACCTGAAGAAGGAGCTGCATG
ACTCAGAAGGCCAGCAGCTTGCTCTCAACAAAGAGATTCTGGTTTTAAAAGACAAATTGGAAAAAACCAG
GAGAGAAAGTCAAAGTGAAAGGGAAGAATTTGAAAATGAGTTCAAACAACAATATGAACGGGAAAAAGTA
TTATTAACTGAAGAGAATAAAAAGTTAACGAGTGAACTCGATAAGCTGACTTCACTGTATGAGAGCTTAA
GTTTGCGCAACCAGCACTTAGAAGAAGAAGTCAAAGATCTAGCAGATAAGAAGGAATCAGTTGCCCACTG
GGAAGCCCAAATCACAGAGATAATTCAGTGGGTGAGCGATGAGAAGGATGCACGAGGCTATCTTCAGGCC
TTAGCTTCTAAAATGACTGAAGAGTTGGAAGCATTAAGAAATTCCAGCTTGGGGACACGAGCAACAGATA
TGCCCTGGAAAATGCGTCGTTTTGCAAAACTGGACATGTCGGCTAGACTAGAGTTGCAGTCTGCTCTGGA
TGCAGAAATAAGAGCTAAGCAGGCCATCCAGGAAGAGCTGAATAAAGTCAAAGCATCTAACATCTTAACA
GAATGTAAACTGAAAGATTCAGAGAAGAAGAACTTGGAACTGTTGTCCGAAATCGAGCAGCTGATAAAGG
ACACTGAAGAGCTTAGATCTGAAAAGGGTATAGAGCACCAAGACTCACAGCATTCTTTCTTGGCATTTTT
GAATACGCCTACCGATGCTCTGGACCAATTTGAACGCTCTCCGTCCTGTACTCCAGCTAGCAAAGGCAGA
CGTATTGCAGACTGTGCTCCACTTCCAGCTCACACACCAACCTTAAGGAAAAAGGGATGCCCGGCTTCAA
CTGGCTTTCCACCTAAGCGCAAGACTCATCAATTTTTCGTGAAGTCTTTCACTGCCCCTACCAAGTGTCA
CCAGTGCACCTCCTTGATGGTCGGTTTGATAAGACAGGGCTGTTCCTGTGAAGTCTGTGGGTTCTCATGT
CATATAACTTGTGTGAACAAAGCCCCGACGGTTTGCCCAGTTCCTCCTGAGCAAACTAAAGGTCCACTGG
GCATAGACCCCCAGAAGGGAGTTGGGACGGCGTATGAGGGTCATGTCAGGATCCCTAAGCCGGCGGGAGT
GAAGAAAGGGTGGCAGAGAGCACTGGCTGTGGTCTGCGACTTCAAACTGTTTCTTTATGATATCGCAGAG
GGAAAAGCATCGCAGCCCACCTCCGTCATTAGTCAAGTGATTGACATGAGAGATGAAGAATTTTCTGTGA
GTTCAGTCTTAGCTTCTGATGTTATTCACGCAAGTCGAAAAGATATCCCCTGTATATTCAGGGTCACAGC
TTCCCAGCTCTCAGCACCTAGTAACAAATGTTCCATCCTGATGCTAGCAGACAGCGAGAATGAGAGGAGT
AAGTGGGTGGGAGTGCTGAGTGAACTCCACAAGATTCTGAAGAAAAACAAGTTCAGAGACCGCTCAGTGT
ATGTCCCCAAGGAGGCGTACGACAGCACGCTACCCCTCATTAAGACAACGCAGGCCGCTGCAATCATAGA
TCATGAACGGATAGCTTTGGGAAATGAAGAAGGATTATTTGTTGTGCATGTCACCAAAGATGAGATTGTT
AGAGTTGGTGATAATAAGAAAATCCATCAGATCGAACTCATTCCAAGTGACCAGCTTGTCGCTGTGATCT
CAGGCCGAAATCGCCACGTCCGACTTTTTCCTATGTCAGCCTTGGATGGGCGAGAGACGGATTTTTATAA
ACTGGCAGAGACTAAAGGGTGTCAGACCATAGCTGCTGGGAAAGTGCGCCACGGAGCTCTGTCCTGCCTG
TGTGTGGCCATGAAGAGACAGGTCCTCTGCTATGAACTGTTCCAAAGCAAGACCAGGCACAGGAAGTTTA
AGGAGATTCAAGTCCCATGTAATGTCCAGTGGATGGCCATCTTCAGTGAGCATCTCTGTGTGGGATTCCA
GTCAGGATTTCTCAGATACCCCCTGAATGGAGAAGGAGGTCCATGCAATATGCTCCATTCAAATGACCAT
ACACTGTCATTCATTTCACACCAACCAATGGACGCTCTCTGTGCAGTTGAGATCTCCAACAAAGAGTACT
TGCTGTGTTTTAACAGCATTGGGATATACACAGACTGCCAGGGCCGAAGATCCAGACAGCAGGAACTGAT
GTGGCCAGCAAATCCTTCCTCCTGTTGTTACAATGCTCCATATCTCTCCGTGTATAGTGAAAACGCAGTT
GATATCTTTGATGTAAACTCCATGGAATGGATACAGACTCTTCCTCTCAAAAAGGTCCGACCTTTAAACA
CTGAAGGATCATTAAATCTCTTAGGATTGGAGACCATTAGATTAATATATTTCAAAAATAAGATGGCAGA
AGGGGATGAACTTGTGGTTCCTGAAACATCAGATAATAGTCGGAAACAAATGGTTAGAAATATCAACAAC
AAACGGCGTTACTCCTTCCGAGTCCCTGAAGAAGAGAGGATGCAACAGAGGAGGGAGATGCTACGAGATC
CAGAAATGAGAAATAAATTAATTTCTAACCCAACTAATTTTAACCACATAGCACACATGGGTCCTGGAGA
TGGAATACAGATCCTAAAAGACCTGCCCATGAACCCACGGCCTCAGGAAAGCCGGACAGTATTCAGTGGC
TCCGTCAGTATTCCCTCCATCACCAAGTCCCGCCCTGAGCCAGGCCGCTCCATGAGTGCCAGCAGCGGCC
TGTCTGCACGGTCGTCAGCCCAGAATGGCAGTGCACTAAAGAGGGAATTCTCTGGAGGAAGCTATAACAC
AAAGCGACAGCCCATGCCCTCTCCCTCGGAGGGCTCTTTGTCATCAGGAGGCATGGACCAAGGAAGTGAC
GCCCCTGCGAGGGACTATGATGGAGAGGACTCTGATTCTCCAAGGCATTCCACAGCTTCCAACAGCTCTA
ACCTGAGCAGCCCCCCAAGTCCCATTTCACCCCAAAAGACCAAGAGCCTCTCCCTGGAGAGCACAGACCG
CGGGAGCTGGGATCCATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001033285
Insert Size 5199 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033285.1, NP_001028457.1
RefSeq Size 8656 bp
RefSeq ORF 5199 bp
Locus ID 226751
Cytogenetics 1 H4
Summary Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LRP35A, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A and LIMK2. May play a role in TFRC-mediated iron uptake (By similarity). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).UniProtKB/Swiss-Prot Function
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"NM_001033285" in other vectors (2)
SKU Description Size Price
MG217902 Cdc42bpa (tGFP-tagged) - Mouse CDC42 binding protein kinase alpha (Cdc42bpa), (10ug) 10 ug
$1,655.00
MR217902 Cdc42bpa (Myc-DDK-tagged) - Mouse CDC42 binding protein kinase alpha (Cdc42bpa) 10 ug
$1,455.00

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