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Kdm6b (NM_001017426) Mouse Untagged Clone

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SKU
MC224778
Kdm6b (untagged) - Mouse KDM1 lysine (K)-specific demethylase 6B (Kdm6b), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
  $1,545.00
8 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Kdm6b
Synonyms 1700064E03Rik; BC038313; Jmjd3; mKIAA0346
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224778 representing NM_001017426
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCTCTGCACGGGAAGCCTTTGCCCTTGGGGGCTTGAGCT
GTGCTGGGGCTTGGAGCTCCTGCCCACCCCATCCTCCTCCCCGAAGCTCATGGCTGCCCGGAGGCAGATG
CTCTGCCAGCGTTGGGCAGCCCCCACTCTCAGCTCCTTTACCCCCATCTCATGGCAGTAGCTCCGGGCAC
CCTAACAAACCCTATTATGCTCCTGGGACACCCACCCCAAGACCCCTTCACGGGAAGTTGGAATCCCTAC
ATGGCTGTGTCCAGGCATTGCTCCGGGAGCCAGCGCAGCCAGGGTTGTGGGAACAGCTTGGACAGTTGTA
TGAATCAGAGCACGACAGTGAGGAAGCCGTATGCTGCTACCATAGGGCCCTTCGCTATGGAGGAAGCTTC
GCCGAGCTGGGACCCCGGATTGGCCGCTTGCAGCAGGCCCAGCTCTGGAACTTTCATGCCGGTTCCTGTC
AGCACAGAGCCAAGGTCCTGCCTCCCCTGGAGCAAGTCTGGAATTTGCTGCACCTTGAGCACAAACGGAA
CTATGGGGCTAAGCGAGGGGGCCCTCCAGTGAAGAGATCTGCTGAACCCCCCGTGGTCCAGCCTATGCCT
CCTGCAGCCCTCTCAGGCCCCTCAGGAGAGGAGGGCCTTAGCCCTGGAGGCAAGCGCAGGAGAGGCTGCA
GCTCTGAACAGGCTGGCCTTCCCCCAGGTCTGCCACTCCCTCCACCACCCCCACCCCCACCGCCTCCACC
ACCACCACCACCCCCTCCACCACCACCGCTGCCTGGCCTGGCTATTAGCCCCCCATTTCAGCTGACTAAG
CCAGGGCTGTGGAATACCCTGCATGGAGATGCTTGGGGCCCCGAGCGCAAGGGTTCAGCGCCGCCAGAGC
GCCAGGAGCAGCGGCACTCGATGCCTCATTCATATCCATACCCAGCTCCCGCCTACTCCGCTCATCCGCC
CAGCCATCGGCTGGTCCCCAACACACCCCTTGGTCCAGGTCCCCGACCCCCAGGAGCAGAGAGCCATGGC
TGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACT
CCAGCGTTTCACCAGCAGCATCTACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGG
CACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGTAACAACACTGGTCTTCGGGGTGTGGAGCCAAGCCCA
GGCATTCCTGGCGCTGACCATTACCAAAACCCTGCGCTGGAGATATCCCCTCACCAGGCCCGCCTGGGTC
CCTCCGCACACAGCAGTCGGAAACCATTTTTGACGGCCCCTGCTGCCACGCCCCACTTATCCCTACCCCC
TGGGACCCCATCATCCCCTCCACCCCCATGTCCTCGCCTCTTGCGCCCTCCACCGCCCCCTGCTTGGATG
AAGGGCTCAGCCTGCCGTGCAGCCCGAGAGGATGGAGAGATCTTAGGGGAGCTCTTCTTTGGTGCTGAGG
GACCTCCCCGTCCTCCTCCCCCACCCCTTCCCCACCGTGATGGCTTCTTGGGGCCTCCAAACCCCCGCTT
TTCTGTGGGCACTCAGGATTCGCATAACCCTCCCATTCCCCCAACCACCACCAGCAGCAGCAGCAGCAGC
AACAGCCACAGCAGTAGTCCTACTGGGCCGGTGCCCTTTCCACCACCCTCCTATCTGGCCAGAAGTATAG
ACCCCCTCCCCAGGCCATCCAGCCCAACCTTGAGCCCCCAGGACCCACCTCTTCCACCACTGACTCTTGC
CCTGCCTCCAGCCCCTCCCTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCTCGGAGAGCCCC
CGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCAGGGGCCACCCCCAGGCCCTGTGAGTAAAG
CCCCCCAGCCTGTGCCACCTGGGGTTGGAGAGCTGCCTGCCCGAGGCCCGAGGCTCTTTGATTTCCCACC
CACTCCGCTGGAGGACCAGTTTGAAGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCAAACATC
ATGAAGATGCTGGATGAATCCATTCGGAAGGAGGAGGAGCAGCAGCAGCAGCAGGAGGCAGGCGTGGCTC
CCCCACCCCCACTCAAAGAGCCCTTTGCATCTCTACAGCCTCCATTTCCCAGTGACACAGCCCCAGCCAC
CACCACTGCTGCCCCCACCACCGCCACCACCACCACAACCACCACCACCACCACCACCCAAGAAGAGGAG
AAGAAGCCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTTCCTCCACCTCCCCAGCCACAGC
CCCCACCACCTCCACCAGCCAGCCCAGCCAGCCTGCTCAAATCGTTGGCCTCTGTTCTTGAGGGACAAAA
GTACTGTTACCGGGGGACTGGAGCAGCCGTCTCAACCAGGCCCGGGTCCGTGCCCGCCACTCAGTATTCC
CCTAGTCCTGCATCAGGTGCTACCGCCCCACCACCCACTTCAGTGGCCCCTAGTGCCCAGGGCTCCCCCA
AGCCCTCGGTTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCTTGGGCCCGGGAGCACAGGGCGGG
TGAAGAGCCAGCACCAGGCCCCGTGACCCCTGCCCAGTTGCCCCCACCTCTGCCGCTGCCCCCTGCTCGT
TCTGAGTCTGAGGTGCTAGAAGAAATCAGTCGGGCTTGTGAGACCCTTGTAGAGCGGGTGGGCCGGAGTG
CCATCAACCCAGTGGACACGGCAGACCCAGTGGACAGTGGGACTGAGCCACAGCCGCCGCCTGCGCAGGC
CAAGGAGGAGAGTGGGGGGGTGGCGGTAGCAGCAGCAGGTCCAGGTAGTGGCAAGCGTCGTCAAAAGGAG
CATCGGCGGCACAGGCGGGCCTGTAGGGACAGTGTGGGTCGACGACCCCGCGAGGGGAGGGCCAAGGCCA
AGGCCAAGGCTCCCAAAGAAAAAAGCCGAAGGGTGCTGGGGAACCTCGACTTGCAGAGTGAGGAGATCCA
GGGCCGGGAGAAGGCCCGGCCCGATGTCGGTGGGGTTTCCAAAGTCAAGACACCCACAGCTCCAGCACCC
CCGCCTGCTCCTGCACCCGCTGCTCAGCCAACACCCCCATCAGCTCCTGTCCCTGGGAAGAAGACTCGTG
AGGAGGCTCCGGGGCCTCCAGGTGTGAGCCGGGCAGATATGCTGAAGCTCCGGTCACTTAGTGAGGGGCC
TCCCAAGGAGCTGAAGATCAGGCTCATCAAGGTGGAAAGTGGGGACAAGGAGACCTTTATCGCCTCTGAG
GTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCCGCCGATGTCATGCGTGCCA
GCAAGAATGCCAAGGTGAAAGGGAAATTCCGAGAGTCCTACCTGTCCCCTGCCCAGTCTGTGAAACCCAA
GATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACCCCCAGCATCTATTTGGAGAGC
AAACGAGATGCCTTCTCGCCGGTCCTGCTACAGTTCTGTACAGACCCCCGGAACCCCATCACCGTCATCA
GGGGCCTGGCTGGTTCACTTCGGCTCAACTTAGGCCTTTTCTCCACCAAGACTCTGGTGGAGGCGAGCGG
TGAACATACGGTGGAGGTCCGTACCCAAGTACAGCAGCCCTCAGACGAGAACTGGGACCTGACAGGTACC
AGACAAATCTGGCCCTGTGAGAGCTCCCGTTCCCACACCACCATCGCTAAATACGCACAGTACCAGGCCT
CGTCCTTCCAGGAGTCACTGCAGGAGGAGAGGGAGAGTGAGGATGAGGAATCCGAGGAACCAGACAGCAC
TACAGGAACCTCTCCCAGCAGTGCACCGGACCCCAAGAACCATCACATCATCAAGTTTGGCACTAACATC
GACCTGTCTGATGCCAAGAGGTGGAAGCCACAGCTACAGGAGCTGCTGAAACTGCCCGCCTTCATGCGGG
TAACATCCACAGGCAACATGCTCAGCCACGTGGGCCACACCATCCTGGGCATGAACACCGTGCAGCTATA
CATGAAGGTCCCTGGCAGCCGAACGCCAGGCCACCAAGAGAATAACAATTTCTGCTCAGTCAACATCAAC
ATTGGCCCTGGGGACTGCGAGTGGTTCGCGGTACATGAGCACTATTGGGAGACCATCAGCGCCTTCTGCG
ACCGGCATGGTGTGGACTACTTGACTGGTTCCTGGTGGCCAATCTTGGATGACCTCTATGCGTCCAATAT
TCCTGTTTACCGCTTCGTGCAGCGCCCTGGAGACCTTGTGTGGATTAATGCAGGGACTGTACATTGGGTG
CAGGCTACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACCAGCTGG
CCCTGGAGCGATATGAGTGGAACGAGGTGAAGAACGTCAAGTCCATTGTGCCCATGATTCATGTGTCCTG
GAACGTCGCTCGAACGGTCAAGATCAGCGATCCTGACTTGTTCAAGATGATCAAGTTCTGCCTCCTGCAG
TCAATGAAGCACTGTCAGGTACAGCGGGAGAGCCTGGTGCGGGCAGGGAAGAAGATCGCTTACCAAGGCC
GTGTCAAAGACGAGCCTGCCTACTACTGCAACGAATGCGACGTGGAGGTGTTCAACATCCTGTTCGTTAC
AAGTGAGAATGGCAGCCGAAACACGTACCTGGTGCACTGCGAGGGCTGTGCGCGCCGTCGCAGCGCGGGC
CTACAGGGCGTGGTGGTGCTAGAGCAGTACCGCACGGAGGAGCTGGCGCAGGCCTACGATGCCTTCACAC
TGGCTCCCGCCAGCACGTCTCGATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001017426
Insert Size 4926 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001017426.1, NP_001017426.1
RefSeq Size 6654 bp
RefSeq ORF 4926 bp
Locus ID 216850
UniProt ID Q5NCY0
Cytogenetics 11 B3
Summary Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).UniProtKB/Swiss-Prot Function
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"NM_001017426" in other vectors (1)
SKU Description Size Price
MR212060 Kdm6b (Myc-DDK-tagged) - Mouse KDM1 lysine (K)-specific demethylase 6B (Kdm6b) 10 ug
$589.00 $1,404.00

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