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Prag1 (NM_172911) Mouse Untagged Clone

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SKU
MC224352
D8Ertd82e (untagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,293.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prag1
Synonyms 9830148H23; D8Ertd82e; mFLJ00269; NACK; Sgk223
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224352 representing NM_172911
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCGTGCAGCGACTTTGTGGAGCACATCTGGAAACCCGGGTCTTGCAAGAACTGCTTCTGCTTGC
GAAGTGATCATCAGCCAACAGCTGGCCATCCCAAGGCTAGAGCCAACAGCCTACCCGCTGGAACTCGCCT
GCCTGCCAGGCCTGAGAATTGCCGCCTAGACGACGAAGGCGTGAATGGCTTGGCCTACTCAAAGCCCACC
ATTGCTGTAAAGCCCACCATGATGACTTCTGAGACCTCCGACTTGTGGACTGAAGCCAGCCTGAGCGCTG
AAGTCCCAAAGGTCAATTGGAGACGAACCCCAGGCAAGCTCCTGCTCCCGAAACAGGAAGATGAGCCAAT
AGTTTACCTAGGCAGCTTCCGAGGACTGCAGAAGCCTGCAAGCCCTCTTGCCTGCACAGATGGCAACTCT
CGTTGTCCCCCAGCCTATACCATGGTCGGACTGCATAACCTGGAGGCCCGCGTGGATAGGAACACTGCCT
TTCAACCAGTGAGCTTCCAGGAAGAGAAGGCTGGGAGGGAAGAGCTGCCCTCAGCTCACGAGAGCTTTCG
CCAGAAGCTGGCTGCCTTTGCAGGGATGACATCCAGCTGCCCCAAGGGCCCCAGGCCCTGCACTTCTCCA
CAGCCCCTGCGGGAATCCCTGCCCTCGGAGGATGACAGTGACCAAAGGTGCTCACCCTCAGGAGACAGCG
AAGGTGGGGAGTACTGTTCCATTCTGGACTGTTGTCCAGAATCCAAAGATGCTGTGCATAGCACCGAGGG
CTCTGGCAGACGTGGAGGGGACTGCTCACCCACGTGCAGGGAGCAGGGACCACGTACAAGACCAACAGAA
GAGGAAAAACAGGGTCTGAGCTTCCCCAGAGAGTGCTGTGGTCAGGGATCCACAGCGAACCCGCCCCGCC
TGGGCCCCAAGAAGCCATCCCTTAACTCTGAGGCCGCCAGCTCTTCCGATGGTCTGTCCTGTGGCAGCAG
CCGCAGCGGGGCCAGCAGCCCCTTCGCTCCCCACCTTGAGAACGACTACTGCTCTCTTGTGAAGGAACCG
GCCTCAGGGAAGCAGCAAGACCTCAGCGGCCACTTTCTCACCTCTGGCAAATGTGTGGGTCAAGCTGCGG
AACTCCAGCCCGCCTCCCTTCTTAGGGACCCTGTGCAGCCTGAACCCATCTACGCCGAAAGCGCCAAGAG
GAAGAAGGCAGCTCCGGGGCCTCCGAGGCCCGAGCCCAAGAAAGAGCAGGTCCCAGCTGGCCATAGCCAG
GGCCAAGTATGGACAGGTGATACCTGGATTCAGAAGACACCCCCTAGCTGGAGCCAGGACAGGGAAGGCG
CAAACCCTGCTCCTCAGGTGGCAACCACCATCACTGTCATCGCTGCTCACCCGGAAGAGGACCACAGGAC
TATTTATCTGAGCAGCCCTGACTCTGCCGTGGGGGTGCAGTGGCCACGTGGGCCTTCGAACCAGGACTTA
CAAGCCGGTGAAGAGGAGCCTTTGGTTGCGCAGGGGCTAACCTCAAGGGAGAGCCATCCACATAATGTGA
CTGAAAACACGGCCAAAGAGAAGCCTGCCATTCCCCCCAAACTGTCCAAAAGCAGCCCCGGAGGGTCCCC
TGTGTCTCCCGCGCCCCCACTGACTGACCACAGCGACGGGAACACCGGTGGCAGCAGCGTCGGACCCCAA
CTTTTGTCTAGAGTCCCTGCTAACCTAACGTCTTCCTGCCACACCAATGGCGTTGCCACTGCTGGTGACT
CTGCCAAGTGTCCCCCACCAGCCACCTCCTCTTCAGTCTTGGACCAGAGGCGGCCAAGATACCAGACTGG
TGCTTGGAGCCGCCAGTGCCGGATAGAAGAGGAGGAGGAGGTGGGGCAGGAGTTGAGTCAAAGTTGGGGG
AGAGAGTTGGAAAACGGTACCGCAGACCATTCAAACTCCTCTACCTGGCACCGTCTCCATCCCATCGATG
GCACCTCTGGGCAGAACAGCAAAACTAACTCTGGGATGAGCAAATCGGCTTCCTTTGCGTTTGAGTTCCC
AAAGGACAGAGGCAGATTGGAGGCATTCTCACCCCCTCCCCCACCTCCAAAGTCAAGGCACCTGTTAAAA
ATGAACAAGAGCAGTTCTGACTTGGAAAAAGTGAGCCAGAGTTCTGCAGAAAGCCTCAGCCCGTCCTTCA
GAGGGGCCCACGTCAGCTTCACCACGGGCTCCACAGATAGCCTAGCCTCGGACTCCAGGCCCTGCAGCGA
TGGAGGTCCATCATATGAACCCACCCACTCACCCACCATCAGCGGGAAAAAGCTCTTCGCTCCCGTCCCT
TTTCCATCAGGCTCCACAGAGGATGTGTCCCCTGGTGGTGGTCCTGCGCAGCCTCCTCCTCTGCCCCAGA
AAAAGATAGTGAGCCGTGCCGCCTCTTCCCCTGATGGCTTCTTCTGGACCCAAGGTTCCCCTAAGCCACG
GACGGCAAGTCCCAAGCTGAACCTAAGCCATTCAGAAACCAATGTTTGTGCCCATGACGAGCCTCCTTTT
AACTGTTCCTTGAACTCTGGAAACCGTTCCCACCATGTCTTCTCCTCTTCTGAGCCTTTGGGGAAAGCTT
TCAAAGGCAATGCTCCCTGGGCCCCAGCTCTGGGGCTGGCGAACAGCAAAGGCGGCTGTGGGAGCCCCAG
TCTCCAGTGCAGAGCGGCCACCTCCACGTCATCATCCCAGCTCAGCGTGTCCAGCCAGGCCTCCTCCAGC
AGCACCCAGCTTCAGCTGCACAGCCTGCTGAGTAGCATCAGCAGCAAGGAGGGCACCTATGCCAAGCTTG
GGGGCCTCTACACACAATCCCTGGCCCGCCTAGTGACCAAGTGTGAAGATCTCTTCATGGGTGGCCAGAA
AAAGGAACTGCGCTTCAATGAGAATTACTGGTCACTCTTCAAGCTGACGTGCAACAAACCTTGCTGTGAC
TCGGGGGATGCAATTTACTACTGTGCCACCTGCTCTGAGGACCCAGGCAGCATCTATGCTGTGAAAATCT
GCAAAACCCCAGAACCCAAATCAGCCTCATACTGCAGCCCATCTGTGCCGGTGCATTTCAACATCCAGCA
AGACTGCGGCCACTTTGTGGCCTCTGTGCCCTCCAGCATGCTTGCCTCCCCTGACACATCCAGCAAAGAC
ACCGCGCCTGCCGTATCCCCACAGCCCCCTGCCCAGGAGCAGGACTGTGTGGTGGTCATCACCCGGGAGG
TACCGCACCAGACTGCCTCCGACTTTGTGCGGGACTCTATGGCCAGCCACCGGGCGGAGCCTGAGGTTTA
CGAGCGCCGGGTGTGCTTCCTGCTTCTGCAGCTCTGCAACGGGCTGGAGCACCTGAAGGAACACGGGATC
ATCCACCGGGACCTGTGTCTGGAGAACCTGCTGCTGGCGCACTGTAACCCTCAGAGCTCCCCCGGGCCCT
CTGCCACTCCTACCGTGCCCACCACCACATCCAGGTGCCCTTCTGCCGCGCCCGCAGCCACCACTGCCTG
CCAGGGAGGGCCTGGTGAGAAGCAGCTGCCCAGGCTCATCATCAGCAACTTCCTGAAGGCCAAGCAAAAG
CCAGGAGGCACCACCAACCTGCAACAGAAGAAGAGCCAGGCCCGGCTGGCTCCCGAGATCGTGTCTGCCT
CCCAGTACCGCAAGTTTGATGAGTTCCAGACGGGCATCCTCATCTATGAGCTGCTCCACCAGCCCAACCC
ATTCGAGGTGCGAGCCCAGCTCCGGGAGCGCGACTACCGGCGGGAGGACTTACCCCCGCTGCCCACACTG
TCCCTCTACTCTCCCGGCCTGCAGCAACTCGCCCATCTACTGCTTGAGGCTGATCCCATCAAGCGCATCC
GCATAGGTGAAGCTAAGCGGGTGCTGCAGTGCTTGCTGTGGGGGCCACGGCGGGAGCTGGTGGAGCAGCC
GTGCACGTCAGAGGAGGTACTGTGTAACACTCTGCACAACTGGATCGACATGAAGCGAGCACTGATGATG
ATGAAGTTTGCTGAGAAGGCGGTGGACCGAAGGCGGGGTGTGGAACTGGAGGACTGGCTTTGCTGCCAGT
ACTTGGCCTCGGCAGAGCCAGGGGCCCTCTTGCAGTCCCTGAAGCTCCTACAACTTCTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_172911
Insert Size 4122 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172911.3, NP_766499.2
RefSeq Size 4780 bp
RefSeq ORF 4122 bp
Locus ID 244418
UniProt ID Q571I4
Cytogenetics 8 22.78 cM
Summary Catalytically inactive protein kinase that acts as a scaffold protein (By similarity). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (PubMed:25038227).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172911" in other vectors (4)
SKU Description Size Price
MG211757 D8Ertd82e (tGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) 10 ug
$1,374.00
MR211757 D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) 10 ug
$589.00 $1,174.00
MR211757L3 Lenti ORF clone of D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) 10 ug
$1,474.00
MR211757L4 Lenti ORF clone of D8Ertd82e (mGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e) 10 ug
$1,474.00

file-alt Citations

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