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Med23 (NM_027347) Mouse Untagged Clone

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SKU
MC224351
Med23 (untagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,290.00
7 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Med23
Synonyms 130kDa; 3000002A17Rik; Crsp3; ESTM7; mKIAA1216; Sur2; X83317
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224351 representing NM_027347
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTACCGATGGAGACGCAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGG
CCTTCCCAGGAATGTTTATGGATACCCCCGAGGATGAAAAAACAAAACTAATTAGCTGCTTGGCGGCCTT
CCGGCAGTTTTGGAGTGGACTTTCTCAGGAGTCTCATGAACAGTGTGTTCAGTGGATTGTTAAATTTATT
CATGGCCAACACAGCCCCAAAAGAATTTCTTTCCTCTATGACTGCTTAGCAATGGCAGTTGAGACTGGTC
TCCTTCCACCCAGGATGGTTTGTGAATCTTTGATAAACTCTGACTCTCTTGAATGGGAAAGAACACAGCT
TTGGGCCTTAACATTTAAGCTGGTTCGGAAAATCATTGGAGGAGTGGATTACAAGGGTGTTCGAGACCTT
CTCAAAGCGATTTTGGAGAAAATCTTGACAATCCCAAATACAGTGAGCTCTGCTGTTGTCCAGCAGCTTC
TGGCAGCAAGAGAGGTTATAGCTTATATCTTAGAACGAAATGCCTGCCTATTGCCGGCCTACTTTGCAGT
CACAGAGATCAGAAAACTATATCCTGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGAC
TTTGTGGATACCTTCAGGCCCACAGCAAGGATTAACTCCATTTGTGGCCGATGCAGTCTTCTGCCAGTTG
TGAACAACTCGGGAGCCATCTGTAACTCATGGAAACTGGACCCTGCAACTCTGCGATTTCCTTTGAAAGG
CCTTTTGCCATATGATAAGGATCTGTTTGAACCGCAGACTGCTTTGTTGAGATATGTACTGGAGCAACCT
TATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTGG
AGGACCAGTTGGTGGACCTGGTGGTGTACGCCATGGAGAGGTCAGAGACCGAGGAGAAGTTTGACGATGG
GGGAACCAGCCAGCTCCTGTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCC
AGTTTCCCACATATGGTCCTCTCTCTCCACCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGGCGAGACC
ATCTGATGTGGGTGCTGCTGCAGTTCATTTCTGGAAGTATTCAGAAAAATGCCCTCGCTGACTTCCTCCC
TGTCATGAAGCTCTTTGACCTGCTGTACCCGGAGAAGGAGTGTATCCCAGTTCCTGATATTAACAAACCC
CAGTCAACGCACGCCTTTGCAATGACTTGTATTTGGATTCATCTTAATAGAAAAGCTCAAAATGGCGACT
CCACGCTCCAGATCCCAATACCCCATTCCCTGAAGCTGCACCATGAGTTCCTCCAGCAGAGTCTGAGAAA
TAAAAGCTTACAGATGAATGACTATAAGATTGCCCTGCTATGTAACGCGTATTCCACAAACTCAGAGTGT
TTTACGTTACCTATGGGAGCTCTGGTAGAAACTATTTATGGAAATGGAATTATGAGAGTGCCTCTCCCTG
GAACGAGCTGTTTGGCCTCAGCATCAGTGACTCCCTTACCGATGAACCTCCTGGATTCACTGACGGTGCA
TGCCAAGATGAGCCTTATTCACAGCATTGCAACCAGAGTGATAAAACTGGCTCATACGAAATCCAGTGTT
GCGCTGGCTCCAGCCCTAGTGGAAACTTACAGCCGTTTACTGGTCTACATGGAAATTGAGTCTTTGGGCA
TCAAAGGATTTATCAGTCAGCTCCTGCCCACTGTCTTCAAGTCCCACGCCTGGGGCATCCTGCACACACT
GCTGGAGATGTTCAGCCACCGAATGCACCACATTCAGCCCCACTACCGAGTTCAGCTCCTGAGCCATCTC
CACACACTGGCTGCAGTCGCACAGACCAACCAGAACCAGCTCCATCTGTGTGTGGAGAGCACTGCACTGA
GGCTCATCACAGCCCTGGGAAGCTCAGAGGTCCAGCCGCAGTTCACGCGTTTCCTCAATGATCCCAAAAC
AGTGCTGTCCGCCGAATCTGAAGAGCTGAACCGAGCCTTGATCCTGACCTTAGCCAGAGCAACCCACGTC
ACAGATTTTTTTACAGGATCTGATTCAATACAGGGAACTTGGTGTAAAGATATCCTTCAGACCATCATGA
ACTTTACTCCTCATAACTGGGCTTCACATACCCTTAGCTGTTTTCCAGCCCCTCTGCAGGCTTTCTTCAA
GCAAAATAACGTGCCCCAGGAAAGCCGTTTTAATCTGAAGAAGAATGTGGAAGAAGAGTACAGGAAGTGG
AAGTCAATGACTGATGAAAACGAGATCATCACCCAGTTCTCTGTGCAGGGCTTCCCTCCACTCTTCCTCT
GTCTCCTCTGGAAGATGCTCCTGGAGACGGATCATATTAGTCAGATCGGCTACAAGGTCCTGGAGAGGAT
TGGGGCCAGGGCCCTGGTGGCCCATGTGAGAACATTTGCAGATTTCCTGGTGTATGAGTTCTCTACGTCA
GCAGGAGGTCAGCAACTCAATAAGTGCATTGAAATTCTCAACGACATGGTGTGGAAGTACAACATTGTCA
CGCTGGACAGACTCATTCTCTGCCTGGCTATGCGTAGTCATGAAGGAAATGAAGCCCAGGTTTGTTATTT
CATAATTCAGTTGCTGTTGCTCAAACCAAATGACTTCAGAAACCGAGTAAGTGACTTTGTGAAGGAGAAT
TCCCCAGAGCACTGGCTCCAGAGTGACTGGCACACCAAGCACATGAGCTACCACAAGAAATATCCCGAGA
AGCTGTATTTTGAAGGCCTGGCAGAGCAGGTTGACCCTCCTGTACCAATCCAGTCCCCTTATCTGCCCAT
CTACTTTGGAAATGTGTGTCTCCGGTTCCTTCCAGTATTTGATATAGTAATCCACAGGTTTTTAGAGTTG
CTTCCAGTGTCCAAATCACTAGAGACTCTCCTGGATCACCTAGGGGGCTTATATAAATTCCATGACCGGC
CAGTGACTTATCTGTATAATACTTTGCACTATTATGAAATGTGCCTGAGAAACCGTGACCATCTCAAACG
AAAACTTGTCCATGCAATCATTGGCTCCCTCAAAGATAACCGGCCACAGGGCTGGTGTCTCAGTGATACC
TACCTGAAGCATGCTATGAATGCACGGGAGGACAACCCCTGGGTTCCGGAGGACTCCTACTACTGCAAGC
TGATTGGCCGACTGGTAGACACCATGGCGGGCAAGTCTCCAGGACCCTTCCCAAACTGTGACTGGAGATT
CAATGAGTTTCCCAACCCCGCTGCCCACGCTCTGCATGTGACGTGTGTGGAACTCATGGCCTTGGCGGTT
CCAGGCAAAGATGTGGGGAATGCCCTTCTCAATGTCGTCCTGAAAAGCCAGCCCTTAGTGCCAAGGGAGA
ACATTACAGCGTGGATGAATGCAATTGGTCTGATCATCACGGCCCTCCCAGAGCCGTACTGGATTGTGCT
TCATGACCGAATTGTGAATGTGATCAGCAGTTCTAGCCTGACGTCAGAGACAGAGTGGGTCGGTTACCCG
TTCCGCCTCTTCGACTTCACCGCTTGTCACCAGTCCTACTCGGAGATGAGCTGCAGCTATACGTTAGCTC
TAGCCCATGCCGTGTGGCACCACTCCAGCATCGGGCAGCTCTCGCTTATCCCAAAGTTTCTCACTGAGGC
GCTTCTCCCGGTGGTGAAGACTGAGTTCCAGTTGCTCTACGTGTACCATCTTGTGGGGCCATTTCTACAG
AGGTTCCAACAGGAGAGAACCCGGTGCATGATTGAGATTGGCGTGGCATTTTATGACATGCTGTTGAATG
TAGACCAGTGCAGCACGCACTTAAATTACATGGACCCCATCTGCGACTTCCTGTATCATATGAAGTATAT
GTTTACTGGTGACAGTGTGAAAGAGCAAGTAGAGAAGATTATCTGTAATTTAAAGCCGGCTTTAAAGCTT
CGTCTTCGATTCATCACGCACATTAGCAAGATGGAGCCGGCAGTGCCACCCCAGGCCCTCAACAGCGGGT
CTCCAGCACCTCAGTCTAACCAGGTGCCAGCATCTCTGCCGGTGACTCAGTGA

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_027347
Insert Size 4113 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027347.3, NP_081623.3
RefSeq Size 4928 bp
RefSeq ORF 4113 bp
Locus ID 70208
UniProt ID Q80YQ2
Cytogenetics 10
Summary Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the pre-initiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data.
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Documents
Resources
"NM_027347" in other vectors (4)
SKU Description Size Price
MG211949 Med23 (tGFP-tagged) - Mouse cofactor required for Sp1 transcriptional activation, subunit 3 (Crsp3) 10 ug
$1,372.00
MR211949 Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 10 ug
$589.00 $1,172.00
MR211949L3 Lenti ORF clone of Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 10 ug
$1,472.00
MR211949L4 Lenti ORF clone of Med23 (mGFP-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 10 ug
$1,472.00

file-alt Citations

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