OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Dgkh (NM_001081336) Mouse Untagged Clone

Be the first to review this product
SKU
MC223782
Dgkh (untagged) - Mouse diacylglycerol kinase, eta (Dgkh), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,108.00
7 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "Dgkh or NM_001081336"

Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dgkh
Synonyms 5930402B05Rik; D130015C16; DGK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223782 representing NM_001081336
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGGCCGGCAGCCAGCACCACCCTCAGGGCGTCGCGGGAGGAGCGGTCGCTGGGGCCAGCGCGG
TTTCCCCCACTGCTGCAGGACCGGGAGAGGATTCGTCCGACAGTGAAGCGGAGCAGGAGGGGCCCCAGAA
GCTGATCCGTAAAGTGTCCACCTCTGGACAGATGCGGACCAAGACTAGTATTAAGGAAGGACAACTGCTG
AAGCAAACCAGCTCTTTCCAAAGGTGGAAGAAGAGATACTTCAAGCTTCGCGGCCGCACACTTTATTATG
CGAAAGACTCCAAGTCCTTGATATTTGATGAAGTTGACCTCTCAGATGCCAGTGTTGCGGAAGCAAGCAC
AAAAAATGCCAACAACAGCTTTACGATCATCACTCCGTTCAGAAGACTGATGCTATGTGCTGAGAACAGG
AAAGAGATGGAGGTCTGGATCAGCTCTCTGAAGTCAGTGCAGAGCAGAGAACCCTATGAGGTGGCCCAGT
TCAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGGCCCACCTTCTGTAA
CGTGTGCAGAGAGAGTCTCTCTGGAGTGACCTCCCACGGCCTGTCCTGTGAAGTGTGTAAGTTCAAGGCT
CATAAAAGATGTGCAGTGAGGGCAACAAACAACTGCAAATGGACCACGCTGGCCTCCATCGGGAAGGACA
TCATAGAGGACGAGGATGGTGTTGCCATGCCTCACCAGTGGCTCGAAGGCAACTTGCCTGTGAGTGCCAA
GTGTGCAGTCTGTGACAAAACCTGTGGCAGTGTGCTTCGCCTGCAAGATTGGAAATGCCTTTGGTGTAAG
ACAATGGTGCACACTGCCTGCAAAGATGTGTACCATCCCGTCTGTCCCCTTGGCCAGTGTAAAGTCTCCA
TCATACCTCCGATTGCACTAAATAGCACAGACTCTGATGGTTTCTGTCGAGCAACCTTTTCGTTCTGTGT
CAGCCCCCTCTTGGTGTTCGTCAACTCTAAGAGTGGAGATAATCAGGGAGTGAAGTTCCTTCGTCGCTTT
AAACAGTTGCTCAACCCAGCTCAGGTGTTTGATTTAATGAACGGAGGGCCTTATTTAGGCTTGAGATTGT
TTCAAAAGTTTGACAACTTTCGGATCCTTGTCTGTGGAGGAGATGGAAGTGTAGGCTGGGTTTTGTCAGA
GATTGATAAACTGAACTTGCATAAACAGTGCCAGCTTGGGGTGCTACCCTTGGGTACCGGCAATGACCTT
GCTCGAGTTCTTGGCTGGGGAGGTTCATATGATGATGACACCCAACTCCCTCAGATACTAGAGAAGCTGG
AACGAGCCAGTACCAAAATGCTGGACAGGTGGAGTATAATGACCTATGAGCTCAAATTGCCAGCAAAGTC
GTCTCTACTTCCTGAACCTGTGGCAGCAACTGAAGAATTTTACATGACAATTTATGAAGACTCCGTTGCA
AACCATCTTACGAAAATCGTCAACTCTGATGAGCATGCGGTGGTCATATCGTCTGCCAAGATACTCTGTG
AAACCGTGAAGGACTTCGTTGCCAAAGTGGAGAAGGCACAGGACAGAACACTGGAAAATACAGTAGTAGC
CGAAGCCGTGGCCAGTAAATGCTCAGTCCTAAACGAGAAGCTTGAGCAGTTGCTACAAGCCTTGCACGCG
GACTCTCAGGCCAGTCGAGTCCCCCCAGGAATCGGCCCTGCCATCCCCGAAGAGGACACCGTGGAGTCCG
CCAGTGACGAGTCCTTGGGTGAAAGCAAGGACCAGCTGGTGAATGACATTGGGAAACCCTCCTCCCAGAA
AGCCGTGAAGCCCCGGGAGATAATGCTGCGGGCCAACAGTCTAAAGAAAGCGGTGAGGCAAGTCATTGAA
GAAGCTGAAAAAGTTATGGATGAGCCAGCAGTGCAGCCCTCTGAGCCAGTCAGTCCGTCTTGTGACTATG
ATACAGAAACAGATGAAGCTAAAGAGGACGACGCGAAAGAGTCGCTATCAGCCAAAACCACATCTCAGTC
TCCTGATGCCCAGGCAAGCTGTGGCCATCCCCAAACTGACTCTGTCGCTGGTCCAGCCATGGCCACCACC
AAAGAAAACCTCCCTGTGCTCAACACTAGAATCATCTGCCCAGGTTTGAGAGCAGGACTTGCTGCCTCCA
TTGCTGGGAGTTCTATCATCAACAAAATGTTGCTGGCGAACATCGATCCTTTCGGGGCAACACCGTTTAT
TGACCCAGATCTAGACTCACTAGATGGATACTCTGAAAAATGTGTCATGAACAACTACTTTGGGATTGGA
TTAGATGCAAAAATTTCATTAGAATTTAATAATAAGAGAGAGGAGCACCCTGAAAAATGCAGAAGTCGAA
CTAAAAACTTGATGTGGTACGGAGTCCTTGGGACCCGGGAGCTACTACAGAGATCGTACAAGAATCTGGA
GCAGAGGGTTCAACTTGAGTGTGATGGGCAGTATATACCTCTTCCTAGTCTACAAGGCATAGCGGTGTTG
AACATCCCTAGCTACGCCGGAGGCACCAACTTCTGGGGCGGAACCAAAGAGGATGATATATTTGCTGCGC
CATCCTTTGATGACAAGATTCTAGAAGTTGTCGCAGTGTTTGACAGTGTGCAGATGGCAGTATCAAGGGT
CATTAAATTGCAGCATCACCGGATAGCACAGTGCCGTACGGTAAAAATCACGATATTTGGCGACGAGGGA
GTCCCAGTGCAGGTGGATGGTGAAGCATGGGTCCAGCCTCCAGGGATCATCAAGATTGTGCACAAAAACA
GAGCTCAGATGCTAACCAGAGACAGAGCCTTTGAAAGCACCCTGAAGTCTTGGGAAGACAAGCAGAAGTG
TGACTCTGGGAAGCCAGTTCTTCGAACCAACCTGTACATCCATCCCGCCCCTGACCTGGCAACAGAGGAA
GTGTCACAGATGCGGCTCTGCTCCCAGGCTGCGGAGGAACTCATCACTAGGATTTGTGACGCAGCCACGA
TTCACTGTCTCCTGGAGCAGGAGCTGGCACATGCAGTGAATGCCTGCTCACACGCCCTGAACAAAGCCAA
CCCACGCTTCCCGGAGAGCCTTACGAGAGACACTGCCACCGAGATAGCCATCAACGTCAAGGCGCTCTAT
AATGAGACAGAGGCTTTGCTAGTTGGCAGGGTCCCTTTGCACTTGGAATCTCCTCACGAAGAGCGGGTGT
CCAGTGCCTTACACTCTGTGGAGATGGAGCTTCAGAAGTTAACGGAGATTCCCTGGCTTTATTACATCCT
CCGCCCCAGTGAGGACGAGGAGCCTCCTCTGGATTGTACCAAAAGAAACAACAAAAGCACAGTCTTTCGC
ATAGTGCCAAAGTTTAAAAAGGAAAAGGCTCAGAAACAGAAAACAAGTTCGCAGCCTGGACCTGGGGATT
CCGAAAGTGGGTCATATGAAGCGAATTCTCCAGGGAATTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001081336
Insert Size 3471 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081336.1, NP_001074805.1
RefSeq Size 3967 bp
RefSeq ORF 3543 bp
Locus ID 380921
UniProt ID D3YXJ0
Cytogenetics 14 D3
Summary Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:27643686). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcripts and orthologous alignments from human (NM_001204504.1).
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001081336" in other vectors (4)
SKU Description Size Price
MG222176 Dgkh (tGFP-tagged) - Mouse diacylglycerol kinase eta (Dgkh), (10ug) 10 ug
$1,206.00
MR222176 Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) 10 ug
$589.00 $1,006.00
MR222176L3 Lenti ORF clone of Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) 10 ug
$1,306.00
MR222176L4 Lenti ORF clone of Dgkh (mGFP-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) 10 ug
$1,306.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products