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Pde3b (NM_011055) Mouse Untagged Clone

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SKU
MC223550
Pde3b (untagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,053.00
7 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pde3b
Synonyms 9830102A01Rik; AI847709
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223550 representing NM_011055
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAAGACGAGCGCGAGCGGGACGCGCCAGCCATGAGGTCCCCGCCGCCGCCGCCAGCCTCGGCCG
CCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCGGCAGGACCCTCC
GCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCGCTCCGCGCCGGG
GCACGCCTCTCGCTCGGCGTCCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGAGGCCCGAGCGCT
GGGCGGCCGCGGCAGCCGGGCTCCGGACGCTGCTGAGCGCCTGCTCGCTCAGCCTCAGCCCGCTCTTCAG
CATCGCCTGTGCCTTCTTCTTCCTCACCTGTTTCCTGACGCGCGCGCAGCGCGGCCCGGGCCGCGGCGCC
GGCTCCTGGTGGCTGCTGGCGCTGCCCGCCTGCTGCTACCTGGGCGACTTCGCGGCGTGGCAGTGGTGGT
CGTGGCTGCGTGGGGAGCCGGCGGCGGCGGGCCGGCTCTGCCTGGTGCTGAGCTGCGTGGGGCTGCTGAC
GCTCGCGCCCCGCGTGAGGCTGCGGCACGGCGTCCTGGTGCTGCTCTTCGCCGGCCTGGTGTGGTGGGTG
TCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGTGCCTGGTCGGGGGCGCGG
GATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTCCCCTCCGCCGCGCTCGGC
GAGTACCGCGGAGGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGCTGCGTGTCGCTGGGAGAA
AGCGCAGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGCCTTGTATTTCCCGAGAAC
AGATGATCCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCAGAATTCTGGAGGTGGAAA
TGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTGCTGATTGACCCAAGCCTT
CCCCCACAAGTCATTTCTTCTCTGCGGAGTATCAGTAGCTTGATGGGTGCTTTCTCAGGTTCCTGTAGGC
CAAAGATTAATTCTTTTACACCATTTCCTGGATTTTATCCCTGCTCTGAAGTAGAAGATCCAGTTGAGAA
AGGAGATCGAAAACTTCACAAGGGATTGAGTGGCAGAACCAGTTTCCCAACTCCACAGCTGAGGAGGAGC
TCTGGAGCCTCATCGTTGCTGACTAATGAGCACTGTTCAAGGTGGGATCGCAGCAGTGGTAAGAGGTCTT
ACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAGTTCAAATCTTTTTACAAT
TCCAAAGCAGAGGTCATCGTCTGTGTCACTGACGCACCATGCAGGTCTGAGAAGAGCTGGTGCTTTGCCC
AGCCACAGTCTTCTGAATTCTTCAAGCCATGTACCAGTGTCTGCTGGCTCTCTAACTAATCGATCACCCA
TAGGATTTCCTGATACCACTGATTTTCTTACTAAGCCAAATATTATTTTACATAGATCACTGGGCAGTGT
ATCAAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAATCTGTGTAGCAGCTGTGGACAC
CAAATACTCAAATATGTTTCAACATGTGAACCCGATGGTACAGACCACCCCAGTGAAAAATCAGGTGAAG
AAGACAGCAGTGTTTTCTCAAAAGAACCATTGAACATTGTGGAAACCCAAGAAGAAGAGACCATGAAGAA
AGCCTGCAGGGAGTTATTTTTGGAAGGTGATAGTCACCTGATGGAAGAGGCACAGCAACCAAATATCGAT
CAGGAAGTGTCACTGGATCCAATGTTAGTAGAAGATTATGATTCATTAATAGAAAAGATGAACAACTGGA
ATTTTCAGATTTTTGAGCTTGTAGAAAAGATGGGAGAAAAATCAGGAAGGATTCTCAGTCAGGTTATGTA
TACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAAGAATTTATGAATTATTTT
CGTGCCTTAGAAAATGGCTACCGGGACATTCCATATCACAATCGTGTGCATGCCACAGATGTCCTACATG
CTGTTTGGTATTTGACAACCCGACCAATTCCTGGCTTACCTCAGATCCATAATAACCATGAAACGGAAAC
CAAAGCAGATTCAGATGGTAGACTTGGTTCTGGACAGATTGCTTACATTTCTTCGAAGAGTTGCTGTATT
CCAGATATGAGTTATGGCTGCCTATCTTCAAACATCCCTGCATTAGAATTGATGGCTTTGTATGTGGCAG
CTGCCATGCACGATTACGATCACCCAGGAAGAACAAATGCGTTTCTAGTGGCTACAAATGCACCTCAGGC
AGTTTTATACAATGACAGATCTGTTCTAGAGAATCATCATGCTGCATCAGCTTGGAATCTGTATCTTTCT
CGCCCAGAGTACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGGTTTCGATTTTTAGTTATAG
AAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCAGAATTCAATGCCAAGGCCAATGA
TGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGCCTCTTGGTCTGCCAGGTGTGCATCAAA
TTAGCAGATATCAATGGCCCAGCAAAAGATCGGGACCTACATTTGAGATGGACAGAAGGCATTGTGAATG
AATTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCCCTTCATGGATCGTTCTTC
TCCTCAACTAGCAAAACTCCAAGAATCTTTTATCACCCACATTGTGGGCCCCCTGTGCAACTCCTATGAT
GCTGCTGGCTTGCTGCCGGGTCAGTGGATAGAAACAGAAGAGGGTGATGATACAGAAAGTGATGATGATG
ATGATGATGATGATGGTGGTGGTGAAGAATTAGATTCAGATGATGAAGAAACAGAAGACAATCTAAATCC
TAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGCCAACTAATGCACCACCTCACTGAAAACCAC
AAGATATGGAAGGAAATCATAGAAGAAGAAGAAGAAAAATGTAAAGCTGAGGGGAACAAACTGCAGGTGG
ATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTTATTGAAGAAGCAGATGAAGAGGAAGAACAAAT
GTTTGAATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_011055
Insert Size 3300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011055.2, NP_035185.2
RefSeq Size 5314 bp
RefSeq ORF 3300 bp
Locus ID 18576
UniProt ID Q61409
Cytogenetics 7 59.46 cM
Summary Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).UniProtKB/Swiss-Prot Function
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"NM_011055" in other vectors (4)
SKU Description Size Price
MG219273 Pde3b (tGFP-tagged) - Mouse phosphodiesterase 3B cGMP-inhibited (Pde3b), (10ug) 10 ug
$1,157.00
MR219273 Pde3b (Myc-DDK-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b) 10 ug
$589.00 $957.00
MR219273L3 Lenti ORF clone of Pde3b (Myc-DDK-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b) 10 ug
$1,257.00
MR219273L4 Lenti ORF clone of Pde3b (mGFP-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b) 10 ug
$1,257.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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