Itga5 (NM_010577) Mouse Untagged Clone

SKU
MC223350
Itga5 (untagged) - Mouse integrin alpha 5 (fibronectin receptor alpha) (Itga5), (10ug)
  $1,468.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Itga5
Synonyms Cd49e; F; Fnra; VLA5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_010577
Insert Size 3162 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010577.4, NP_034707.5
RefSeq Size 4440 bp
RefSeq ORF 3162 bp
Locus ID 16402
UniProt ID P11688
Cytogenetics 15 58.9 cM
Summary The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain, which is proteolytically processed to generate light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signaling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. Homozygous knockout mice for this gene exhibit embryonic lethality. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015
Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).
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"NM_010577" in other vectors (4)
SKU Description Size Price
MG211547 Itga5 (tGFP-tagged) - Mouse integrin alpha 5 (fibronectin receptor alpha) (Itga5) 10 ug
$1,575.00
MR211547 Itga5 (Myc-DDK-tagged) - Mouse integrin alpha 5 (fibronectin receptor alpha) (Itga5) 10 ug
$1,375.00
MR211547L3 Lenti ORF clone of Itga5 (Myc-DDK-tagged) - Mouse integrin alpha 5 (fibronectin receptor alpha) (Itga5) 10 ug
$1,675.00
MR211547L4 Lenti ORF clone of Itga5 (mGFP-tagged) - Mouse integrin alpha 5 (fibronectin receptor alpha) (Itga5) 10 ug
$1,675.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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