Ofd1 (NM_177429) Mouse Untagged Clone

SKU MC223219

Ofd1 (untagged) - Mouse oral-facial-digital syndrome 1 gene homolog (human) (Ofd1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Ofd1
• Synonyms: Cxorf5; DXGgc7e; ORF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223219 representing NM_177429; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGATGGCTCAGTCCAACATGCCCCACAAGTCTGATGTGTTGAGTCAAGATGAACTGCGCAAAAAGC; TATACCAGACGTTTAAGGATCGGGGTGTACTGGACACACTTCAGACCCAACTCCGAAACCAGCTAATTCA; TGAATTGATGCATCCTGTATTGAGTGGAGAAGTGAAGCCGCCATCCATTTCAGTGGAAGGGAGTGCCCTC; CTAATAGGGGCTTCTAACTCGCTGGTGGCAGACCACTTACAAAGATGTGGCTATGAATATTCACTCTCTG; TTTTCTTTCCAGAAAGTGGTTTGGCAAAAGAAAAGATATTTACTATGCAGGATCTACTACAACTCATTCG; GATTAACCCCTCATCCAGTCTCTATAAATCATTGATTTCAGGATTTGATAAAGAAAATAAAAAAGGTTTT; CTTATGAGTTTCTTAAAGGAGTTGGCAGAATATTATCAAGCTAAAGAGAGCTGTGATGCGGAAACTCAGA; CAAGCACCACATTTCCCAGCCAAGTGTCCCTAGCCGAGAAGTTTCAGCTTATTGACGCTCAATTTGCAGA; TGGTTTTCCTCACCGTTCCAAGCTAGAATCTTTAGAGACAAAGTTAAACGAGTATAAGAAAGAAGTACAA; CACCAGCTTCAGGTAGAAATGTGTCATAAGCTGAAGTATTTTAGAGAAGCTGAAATAACAAAAGTTAAGA; TGGAAGAGAGAAGAAAGTATGAAAAGGAATTGGCTGAGTTCCAGAATGAATTTGAAAGAACTTGTCAAGC; AAAAAATGAAGCCCTCATTTCTCAGGAGAAGAATTCTCTGGAAAGAATTAAAAAACACCGAGAGATGGAA; TCAAAAGAAATTTATGCTCAAAGACAGCTTCTACTAAATGATATAGCTTTGCTTAGAGGTAGAGAAGCAG; AGCTGAAGGAGCGAATCGAAACTTTTGAATTGACCCAGAAGCTCCAAGAAGAAAAAATTAAAAGTGAGGC; TGAGGCACTTGAGAGACGGGAACAGAATCTGAAGAATATTGAAGACACCTATGATCAGAAACTCAAAACT; GAACTGCTAAAGTACCAACTGGAGCTAAAGGATGACTACATCACTAGGACAAATAAACTCCTGGAAGAGG; AAAGGAAGAATAAAGAAAAAACTATTCATTTGCAAGAGGAGCTCACAGTTATTAATTCAAAAAAGGAAGA; ACTCAGTAAATCTGTTAAGCATATGAAAGAAGTTGAGCTGGAACTGGAGTCTGTGAAAGCTCAGTTTTTA; GCAATATCAAAACAAAACCACTTGCTGAATGAAAAGGTGAGAGAGATGAGTGATTACTCACAACTGAAAG; AAGAGAAAGTGGAGCTTCAGGCACAAAATAAACTACTTAAACTGCAACTGGAAGAGACTAGGAATGAAAA; CTTACGACTCCTAGACCGCATAACTCAGCCACCTCCTGAGCTTGTGATTTTTCAGAAGGAATTACAGAAA; ACAGAAAAGGCTATGGAACTGGAGCACAAGGACTTTGAAACTCATAGGCAGGCTCTTGAGAAACAGCTGC; AGAGTGAAATTGAAAATTCTGCACAGCTAAGGACCCAGATAGCAGAATATGATGCTTCTGTCAAAAGGTT; AACTGTTCAGGTGGCTGAGTTAAAGTCACAACTAAAACAAACTCAGATAGCCCTAGAGAATGAAGTGTAC; CGAAATCCAAAACACTCTCTGATTCATTCCCTGAGTGGCCTCCTATTGAGCGGCAAGATGGCACCCCACA; GTGAAGATAAAAGTGGGGATTTCTTGAATGTACCCCTTGAACAGAACAAGGTTATAGCAGGTGCAGTTAT; GTCAAGGGTCCCACCTTATGTAAATACAGCAACAGAAGCTAGTTCCCCAGAGTCTGACTTTGAGTTTATA; GCCAGTAGTACTAAAGCCAAAGTAAGAGAGCTAGAGCAAGAGGCTGAACGCTTGGAAAAGGCATTCAGAA; CTTACTATCAAAGAGCTACTCAGAATCCTTCTACGAGCCCTCAGCCGGCAAAGAGTCCTCCATCTGTAAA; CTCGGTGGCAGCTCTCAGAAGCATAGCTTCCAGTTCCATGGACAGACCTGTTTCTGCAGAGGACAGAGTT; GTCTCGGAACAGCCTCTTGGGGACATGCTGAAGGAAGAAATGAGTGATATGTCCAAAGCATTCATGGGCA; GTGTGGTTTCACGGCCACGCAGGACCTCTTCCTCCACACGCCTCTCCTCTACACCCCATCCAAAATCGAG; AAGAAGTCTTGATAATGAAATGTATCTGGAAGGTCTCGGGAGATTACACATGACTTCCTCCAGCCCTCTT; CTTGACAGAGTTTCTGCATCACCTGCTGCTTCCCCTAGTCCTTGTCCTGAGAGAACAGCCCAGGCGTCAC; CTGTTCCATCTAGGCACAGCTTCTCTGGTCTTCCAGAGCAAAATGCCTGTTTATATCAGAGACAAACTGA; AACGCAAGATAAAAGTGAGCTTTCAAATGTGGACAAGCAATCTTTGAAAGATGAGAAATTTGAACCACCT; TTCAGATGGAACAAGACAGAACAGTTTGAAGCAGAAGGACTCCACCCTGCTGGTGACATGCCTGGAATAG; ATTTTGCTGTGGCTACTCAGTCTTCAAGACTCATTTCATACGACTACCCAAGTGCTGTTCAGAGCCAAAC; TGGAGAACAAGACGAACAGGAGCTATGGGAGCTTCACATGAAAGAACGCAGGCAGAGAGAAGAACAAAGG; CATAATGAACGGCAAGAAGCTTTAGAGAGGGAAAGAAGAGAGCTGGGAAAACTAGAGCAGGAGCGGAGGA; TGATTGAAGAGTCACTGAAGATGGAGATGGAAGAAGAATTAGAAAAGAGTGTTCAAGACCAGAAAGACAA; ATCAGCTCATTGTGAAAATACTTTAGAAAAATACATGAAAATTATCCAGCAGAGACAGGAAGAATCAAAT; GCAGATAAGAGCTCCAAAAAATCGGGCAAAGAATGCTCACTAGTGGACATGATGATGCCTAGTGACAAGG; ATGAAAGTTCTCCAGGATTTTCTCATGAAGAACCAGATGACATGTGGTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177429
• Insert Size: 3060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177429.3, NP_803178.2
• RefSeq Size: 4453 bp
• RefSeq ORF: 3060 bp
• Locus ID: 237222
• UniProt ID: Q80Z25
• Cytogenetics: X 77.28 cM
• Summary: Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation.[UniProtKB/Swiss-Prot Function]