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Vps54 (NM_139061) Mouse Untagged Clone

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SKU
MC222927
Vps54 (untagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $985.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Vps54
Synonyms Hcc8; mSLP8; Vps54l; wr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222927 representing NM_139061
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAAGCCACAGTTCTTCTCCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAAAAAGAGG
TAGATCCAACAAAACACATTCGACCTGTGCAGTCACTGCCAGATGTGTGTCCCAAGGAACCCACAGGTGA
TTCACATACTTTATGTGTTGCCCCATCTCTAGTTACAGATCAACATAGATGGACTGTGTATCATTCCAAA
GTAAATCTCCCAGCAGCATTAAATGATCCTACACTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACTT
GGGGATTGGACTTTGTGGACACTGAAGTCATACCTTCACTCTACCTCCCACAGATCAGCAAGGAGAATTT
TATAGCATATCAGCAGGAGATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCT
AAAGATACCTTTGACAGGACTCTTTTACATATTCATGATAAATCCAGGACAGATCTGGAGCAAGTACCTA
AGATTTTTATGAAACCAGATTTTGCCTTGGATGATTCTTTAACTTTTAATTCAGTTTTACCATGGTCTCA
TTTTAATACTGCTGGTGGGAAAGGAAGTCGCGACGCAGCTTCTTCCAAGTTGCTTCAGGAAAAGCTGAGC
CATTATCTGGATATTGTGGAAGTAAACATTGCTCACCAGATCTCTCTACGTTCAGAAGCATTTTTTCATG
CAATGACCTCTCAACACGAATTGCAGGACTACCTCAAGAAAACTACCCAGGCTGTAAAAATGCTTCGAGA
TAAAATTGCACAGATTGATAAAGTTATGTGTGAAGGATCACTACAGATTTTAAGACTGGCACTTACCAGA
AATAATTGTGTTAAAGTATACAATAAACTGAAGTTAATGGCCACTGTACACCAAACTCAGCCTACAGTGC
AGGTGTTGTTATCTACTTCTGAATTTGTTGGAGCGTTGGACTTAATAGCAACAACTCAAGAGGTTCTGCA
GCAGGAGCTTCAGGGCATTCACAGCTTCCGGCATTTGGGATCACAGCTTTGTGAATTAGAAAAGCTGATT
GATAAAATGATGATTGCAGAATTTTCTACTTATTCTCATAGTGACTTAAATAGACCACTGGAAGGTGAAT
GCCAAGTTTTAGAAGAGGAAAGATTAGTATCTCTTGTATTTGGGCTTTTGAAACAAAGAAAACTTAATTT
TTTAGAAATCTATGGTGAAGAAATGATTATTACAGCAAAGAACATCATTAAAGAGCGTGTGATTAATAAA
GTTTCACAAATAGAAGAAATAGACACAGACGTTGTTGTAAAGCTTGCAGATCAAATGAGAATGTTGAATT
TTCCCCAGTGGATTGATCTGTTAAAGGACATTTTCTCTAAGTTTACAGTTTTCCTACAGAGAGTTAAGGC
AACATTGAATATTATCCACAGTGTTGTTCTGTCAGTTCTTGAGAAAAGCCAAAGGACTAGAGAATTGGAA
GAGATCCCACAGCAGAGGAGCGCTGGGAAGGACAGCTCCTTGGACACAGACGTGGCTTACCTGACACACG
AGGGCTGGTTCATAAGTGATGCTTTCAGTGAGGGTGAGCCAGCATCTGCGGCGGTTGATACTACCTCTCA
AAGAAACACGTCTCCACATAGCGAGCCCTGCAGCAGTGATTCGGTATCAGAACCAGAATGTACTACTGAT
TCTTCATCCAGCAAGGAGCAGACATCAGCGTGTGCTCCTCCAGGAGGTATAGAGATTATAGTCAGTGAAG
ACATGAGGTTAACTGACTTGGAGCTAGGAAAACTAGCAAGTAACATCCAGGAGTTACTGTGTAATGCTTC
AGATGTATGCCATGATCGAGCTGTCAAATTTCTCATGTCAAGAGCAAAGGACGGTTTTCTTGAAAAATTG
AATTCCACGGAATTCATAGCACTCTCGAGATTAATGGAGACATTCATAGTGGACACTGAACAGATCTGTG
GGAGGAAGAGCACGTCACTGCTTGGGGCCCTTCAGAGCCAAGCAAATAAGTTTGTGAACAGGTTTCATGA
AGAGAGGCGGACCAAGCTCAGCCTCCTCTTAGACAACGAGCGTTGGAAGCAGGCTGATGTTCCTGCAGAA
TTTCAGGATCTTGTTGATTCTATAGCGGATGGAAAGATTGCTTTACCTGAAAAAAAGCCAGTGGTTACAG
AAGAAAGGAAGCCAGCTGATGTTCTTGTTGTTGAGGGACACCAGTATGCAGTTGTCGGAACCGTCTTGCT
GTTAATAAGAATCATTCTTGAATATTGCCAGTGTGTGGATAACATCCCCTCCGTCACTACTGACATGCTC
ACTCGCCTGACAGATCTGCTGAAGTACTTCAATTCAAGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCTT
TGCAAGTTGTTGGACTAAAAACAATTACTACAAAAAACTTGGCCCTCTCTTCACGGTGTCTGCAGTTGAT
TGTGCACTACATTCCCGTGATCCGGGCTCACTTTGAAGCCCGACTGCCCCCCAAGCAATGGAGCATGCTT
AGACATTTTGATCACATCACAAAGGATTACCATGATCATATAGCTGAAATTTCAGCTAAGCTTGTAGCGA
TAATGGATAGCCTATTTGATAAGCTGTTATCAAAGTATGAGGTGAAAGCTCCTGTTCCTTCCCCGTGTTT
CAGGAATATTTGTAAGCAAATGACAAAAATGCATGAAGCTATATTTGATCTTCTTCCCGAAGAACAAACA
CAGATGTTATTTTTAAGAATTAATGCAAGTTACAAACTCCACTTGAAAAAACAGTTATCACACTTAAATG
TAATAAATGACGGAGGACCTCAAAATGGGTTGGTCACAGCAGATGTAGCTTTTTACACTGGAAATCTTCA
AGCCTTAAAAGGCCTTAAAGATCTGGATCTAAATATGGCCGAGATCTGGGAACAGAAGAGGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_139061
Insert Size 2934 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139061.5, NP_620692.3
RefSeq Size 4569 bp
RefSeq ORF 2934 bp
Locus ID 245944
UniProt ID Q5SPW0
Cytogenetics 11 13.89 cM
Summary Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_139061" in other vectors (4)
SKU Description Size Price
MG227283 Vps54 (tGFP-tagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54), (10ug) 10 ug
$1,094.00
MR227283 Vps54 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54) 10 ug
$589.00 $894.00
MR227283L3 Lenti ORF clone of Vps54 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54) 10 ug
$1,194.00
MR227283L4 Lenti ORF clone of Vps54 (mGFP-tagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54) 10 ug
$1,194.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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