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Edem3 (NM_001039644) Mouse Untagged Clone

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SKU
MC222691
Edem3 (untagged) - Mouse ER degradation enhancer, mannosidase alpha-like 3 (Edem3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,365.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Edem3
Synonyms 2310050N11Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222691 representing NM_001039644
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAAAGCCGGCGGCTGCCGGGGCTGTGGGTGCCGGGTCCCCCAGCGAGCGTCATGGAGCCTGGTGG
CAGCGACGGCCGCGCTCTGTCTGGTGTTGGCCACGTCCGTGTGCACGGCAGGGGCCGCGCCCATGAGTAG
GGAAGAGAAGCAGAAGCTTGGAAATCAAGTCCTGGAAATGTTTGATCATGCTTATGGCAACTATATGGAA
CATGCCTACCCAGCTGATGAGCTCATGCCTTTAACCTGCCGGGGCCGTGTCAGAGGCCAGGAGCCCAGCC
GGGGCGATGTGGATGACGCCTTGGGAAAGTTTTCCCTAACACTGATTGATTCTTTGGACACTCTTGTGGT
ATTAAACAAAACTAAGGAATTTGAAGATGCTGTGAGAAAAGTTTTAAGGGATGTTAATTTAGATAATGAT
GTAGTTGTTTCAGTCTTCGAAACAAACATCAGAGTTCTTGGGGGTCTTTTGGGCGGACACTCGCTGGCCA
TCATGCTGAAGGAGAAAGGCGAGCACATGCAGTGGTACAATGACGAGCTCCTCCACATGGCAAAGCAGCT
GGGTTACAAGCTCCTGCCAGCTTTCAACACCACCAGCGGCCTTCCGTACCCCAGGATTAATTTAAAGTTT
GGCATCAGAAAACCAGAAGCCAGGACGGGAACAGAGACAGACACCTGTACAGCCTGTGCAGGCACCTTGA
TCCTCGAGTTTGCTGCACTGAGTCGATTCACAGGAGCGACCATATTTGAGGAATATGCAAGAAAAGCTCT
TGATTTTCTCTGGGAGAAAAGACAGCGAAGTAGCAATTTAGTAGGCGTGACTATCAATATTCATACTGGA
GACTGGGTACGCAAAGATAGTGGAGTTGGAGCGGGGATCGACTCCTATTATGAGTATCTGTTGAAAGCCT
ATGTCTTGCTTGGAGATGACAGTTTTCTGGAAAGATTTAATACACATTACGATGCCATAATGAGGTATAT
AAGCCAGCCACCTCTCCTACTTGATGTGCATATCCACAAACCAATGCTGAACGCTCGGACCTGGATGGAT
GCATTGCTTGCCTTTTTTCCAGGTTTGCAGGTTTTAAAGGGTGATATTAGACCTGCTATTGAAACTCACG
AAATGTTATACCAGGTGATTAAAAAACACAATTTTCTACCAGAGGCATTTACTACAGATTTCAGGGTCCA
TTGGGCTCAGCATCCTTTAAGGCCAGAGTTTGCAGAAAGTACCTACTTCTTATATAAGGCTACAGGAGAC
CCTTACTACCTTGAAGTAGGGAAAACACTTATTGAAAATTTGAATAAGTATGCTCGAGTGCCTTGCGGGT
TTGCTGCCATGAAGGATGTTCGCACTGGGAGTCATGAGGACAGAATGGATTCATTCTTCTTGGCTGAAAT
GTTTAAATACCTGTACCTGTTATTTGCTGATAAAGAGGACATCATTTTTGACATAGAAGACTATATCTTT
ACAACAGAAGCTCATCTGTTACCGCTTTGGCTTTCCACCACAAACCGAAGCATCTCAAAGAAGAACACAA
CCTCAGAATACACAGAACTGGATGATAGTAATTTTGATTGGACTTGTCCAAACACTCAGATTCTTTTTCC
TAATGATCCATTGTATGCTCAGAGCATCCGTGAACCCTTGAAAAACGTGGTGGATAAGAGCTGTCCGAGA
GGCATCATCAGAGTAGAGGAGAGTTTCAGAAGTGGAGCTAAGCCTCCTCTGAGAGCCAGAGATTTTATGG
CCACTAACCCTGAACATTTAGAAATCCTGAAGAAGATGGGGGTGAGTTTGATTCACCTCAAAGATGGGCG
AGTCCAGCTGGTCCAACATGCAATCCAAGCTGCTAGTTCAATTGATGCTGAAGATGGGTTACGGTTCATG
CAGGAGATGATAGAGCTGTCCAGTCAGCAACAGAAGGAGCAGCAGCTGCCTCCACGAGCTGTCCAGATTA
TCTCCCACCCGTTTTTTGGCAGGGTCGTCTTAACTGCTGGACCAGCTCAGTTTGGGCTGGATCTATCTAA
ACACAAAGAGACACGAGGATTTGTTGCAAGCAGTAAACCATATAATGGTTGTTCAGAGCTCACTAACCCT
GAGGCAGTGATGGGAAAAATTGCCTTGATACAAAGAGGACAGTGCATGTTTGCAGAAAAGGCACGCAACA
TTCAGAATGCTGGCGCCATCGGTGGCATTGTGATCGATGACAACGAGGGGAGCAGTAGCGACACGGCCCC
CCTGTTCCAGATGGCCGGCGATGGGAAGGACACAGACGACATCAAGATCCCCATGCTGTTCCTGTTCAGC
AAGGAAGGCAGCATCATCCTGGACGCCATCCGCGAGCACGAGCAGGTGGAGGTGCTCCTTTCTGACAAAG
CGAGAGACCGAGATCCCGAAATGGAAAATGAAGACCAGCCATCTTCTGAAAATGATTCTCAGAATCAGAG
CGCAGAGCAGATGCTCTCGCTTTCTCAGACAGTCGACTTGGCTGATAAGGAGAGCCCTGAACACCCTGCA
GATTCTCACTCAGAGGCGTCTCCATCGGACAGTGAAGAGGCTGCTGGCTTTGCCCCCTCTGAACAGATAT
CTGGGTCCACCGAAAACCACGAGACTACAAGCCTTGATGGTGAATGTACAGACTTAGATAACCAAGTTCA
AGAACAATCAGAAACTGAGGAAGATTCCAGTCCTAATGTCAGCTGGGGTACAAAGGCCCAGCCTATAGAC
TCCATATTAGCAGACTGGAATGAAGACATAGAAGCATTTGAAATGATGGAGAAGGATGAACTATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001039644
Insert Size 2796 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039644.2, NP_001034733.2
RefSeq Size 6604 bp
RefSeq ORF 2796 bp
Locus ID 66967
UniProt ID Q2HXL6
Cytogenetics 1 G1-G2
Summary Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes, by catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2 in the N-glycans. Seems to have alpha 1,2-mannosidase activity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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"NM_001039644" in other vectors (4)
SKU Description Size Price
MG217624 Edem3 (tGFP-tagged) - Mouse ER degradation enhancer mannosidase alpha-like 3 (Edem3), (10ug) 10 ug
$1,478.00
MR217624 Edem3 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 3 (Edem3) 10 ug
$1,278.00
MR217624L3 Lenti ORF clone of Edem3 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 3 (Edem3) 10 ug
$1,578.00
MR217624L4 Lenti ORF clone of Edem3 (mGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 3 (Edem3) 10 ug
$1,578.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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